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Molecular Tools and Techniques in Biotechnology: Restriction Enzymes, Gel Electrophoresis, Plasmids, and Genetic Engineering

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Introduction to DNA-Based Technology

  • Map of DNA-Based Technology Lesson
    03:12
  • Introduction to DNA-Based Technology
    02:30

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

Gel Electrophoresis

  • Gel Electrophoresis
    07:58
  • Gel Electrophoresis Example 1
    02:34

DNA Fingerprinting

  • Short Tandem Repeats (STRs)
    03:41
  • DNA Fingerprinting
    03:30

Introduction to DNA Sequencing

  • Introduction to DNA Sequencing
    02:13
  • Dideoxy Nucleotides
    05:12

Dideoxy Sequencing

  • Chain-Termination PCR
    07:37
  • Dideoxy Sequencing
    01:03
  • Determining the DNA Sequence from a Gel
    06:28

Carbon

  • Carbon is an Excellent Building Block
    03:35
  • Carbon
    04:19

Functional Groups

  • Functional Groups Example 1
    02:00
  • Functional Groups
    06:04

Introduction to Biomolecules

  • Introduction to Biomolecules
    01:17
  • Map of the Lesson on Biomolecules
    01:29

Monomers & Polymers

  • Building & Breaking-Down Polymers
    04:19
  • Monomers & Polymers
    03:42

Proteins

  • Protein Structure
    08:02
  • Amino Acids
    04:37
  • Proteins
    02:50

Nucleic Acids

  • Nucleic Acids Example 2
    01:05
  • Formation & Breakdown of Nucleic Acids
    04:07
  • DNA vs. RNA
    05:57

Prokaryotic & Eukaryotic Cells

  • Recap: Prokaryotic vs. Eukaryotic Cells
    04:26
  • Prokaryotic & Eukaryotic Cells
    05:54
  • Features of Bacterial Cells
    03:54

Introduction to Eukaryotic Organelles

  • Introduction to Eukaryotic Organelles
    03:45
  • Ribosomes
    06:51
  • Map of the Lesson on Eukaryotic Organelles
    02:43

Endomembrane System: Protein Secretion

  • Golgi Apparatus
    06:02
  • Endoplasmic Reticulum
    06:07
  • Nucleus
    06:32

Endomembrane System: Digestive Organelles

  • Central Vacuole
    03:35
  • Lysosomes & Peroxisomes
    05:38

Mitochondria & Chloroplasts

  • Chloroplasts
    03:16
  • Chloroplast Structure
    03:41
  • Mitochondria Structure
    04:45

Endosymbiotic Theory

  • Endosymbiotic Theory
    06:58

Introduction to Energy

  • Thermodynamics: System vs. Surroundings
    04:21
  • Introduction to Energy
    04:58

Chemical Reactions

  • Chemical Reactions
    01:56
  • Types of Chemical Reactions
    05:48

ATP

  • Energy Coupling
    04:57
  • ATP
    06:43
  • Phosphorylation
    03:

Enzymes

  • Functions of Enzymes
    02:32
  • Enzymes Example 1
    02:25
  • Enzymes
    02:55

Introduction to Metabolism

  • Introduction to Metabolism
    02:17
  • Catabolic & Anabolic Pathways
    03:25

Redox Reactions

  • Redox Reactions Example 1
    01:44
  • Electron Carriers: NADH & FADH2
    05:22
  • Redox Reactions
    04:47

Introduction to Aerobic Cellular Respiration

  • Introduction to Cellular Respiration Example 2
    01:53
  • Aerobic Cellular Respiration is a Redox Reaction
    04:18
  • Stages of Aerobic Cellular Respiration
    02:31

Glycolysis

  • Phases of Glycolysis
    06:20
  • Glycolysis Example 1
    01:29
  • Remembering Phases of Glycolysis
    02:49

Pyruvate Oxidation

  • Pyruvate Oxidation
    04:38

Krebs Cycle

  • Krebs Cycle Example 1
    01:20
  • Krebs Cycle
    01:37
  • Phases of The Krebs Cycle
    09:54

Electron Transport Chain

  • Remembering the ETC
    04:43
  • Electron Transport Chain
    07:41

Chemiosmosis

  • Chemiosmosis
    05:01

Review of Aerobic Cellular Respiration

  • Total Products for Each Stage of Aerobic Cellular Respiration
    07:25
  • Review of Aerobic Cellular Respiration
    07:51

Fermentation & Anaerobic Respiration

  • Lactic Acid Fermentation
    03:34
  • What Happens to Aerobic Organisms if There's No Oxygen?
    07:51
  • Alcohol Fermentation
    02:02

Genetic Code

  • How to Use the Genetic Code
    11:39
  • Genetic Code
    01:28

Central Dogma

  • Central Dogma
    05:45

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • 3) Termination of Transcription
    02:44
  • 1) Initiation of Transcription
    04:16
  • 2) Elongation of Transcription
    04:01

Eukaryotic RNA Processing and Splicing

  • Eukaryotic RNA Processing and Splicing
    02:32
  • 2) RNA Splicing Creates Mature mRNA
    08:44
  • 1) RNA Processing
    04:19

Introduction to Types of RNA

  • Introduction to Types of RNA
    07:20

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • 2) Elongation of Translation
    04:59
  • 3) Termination of Translation
    02:16
  • 1) Initiation of Translation
    05:03

Post-Translational Modification

  • Post-Translational Modification
    04:26

Review of Transcription vs. Translation

  • Review of Transcription vs. Translation
    04:07

Mutations

  • Mutations
    02:29
  • Types of Mutations
    08:44

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Glucose's Impact on Lac Operon

  • Positive Control by cAMP & CRP
    08:32
  • Glucose's Impact on Lac Operon Example 1
    06:20
  • GlucoseLevels, cAMP, & the Lac Operon
    05:58

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Review of the Lac Operon & Trp Operon

  • Review of the Lac Operon & Trp Operon
    06:24

Introduction to Eukaryotic Gene Regulation

  • Map of Eukaryotic Gene Regulation
    04:22
  • Introduction to Eukaryotic Gene Regulation
    03:47

Eukaryotic Chromatin Modifications

  • Eukaryotic Chromatin Modifications
    03:56
  • Histone Acetylation
    04:14
  • DNA Methylation
    03:39

Eukaryotic Transcriptional Control

  • Introduction to Transcription Factors
    03:32
  • General Transcription Factors
    03:51
  • Specific Transcription Factors
    03:57

Eukaryotic Post-Transcriptional Regulation

  • Eukaryotic Post-Transcriptional Regulation
    01:56
  • 3) RNA Interference
    04:16
  • 1) Alternative RNA Splicing
    05:17

Eukaryotic Post-Translational Regulation

  • Protein Ubiquitination
    02:45
  • Eukaryotic Post-Translational Regulation
    03:55

Viruses

  • Virus Structure
    05:
  • Lytic and Lysogenic Cycles
    08:05
  • Viroids and Prions
    04:22

Genomes

  • Gene Families
    02:06
  • Transposable Elements and Pseudogenes
    03:57
  • Protein Encoding Genes
    02:11