Provide one example of a CRISPR-Cas application for biotechnology.

There are a variety of circumstances under which rapid results using multiple markers in PCR amplifications are highly desired, such as in forensics, pathogen analysis, or detection of genetically modified organisms. In multiplex PCR, multiple sets of primers are used, often with less success than when applied to PCR as individual sets. Numerous studies have been conducted to optimize procedures, but each has described the process as time consuming and often unsuccessful. Considering the information given in Problem 30, why should multiplex PCR be any different than single primer set PCR in terms of dependability and ease of optimization?

What is a single guide RNA, and what role does it play in CRISPR-Cas genome editing in eukaryotic cells?

What is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR) in the context of genome editing?

What safety considerations must be taken before CRISPR-Cas is used to edit human embryos to cure disease?

The purpose of polymerase chain reaction is to do what?

Which of the following lists the steps of genetic cloning in the proper order?

How can gene knockouts, transgenic animals, and gene editing techniques be used to explore gene function?

What experimental evidence confirms that we have introduced a useful gene into a transgenic organism and that it performs as we anticipate?