Domanda del libro di testo
Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?
a. AAGG TTCC
b. GGCC CCGG
c. ACCA TGGT
d. AAAA TTTT
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Ch. 20 - DNA Tools and Biotechnology
Urry12th EditionCampbell BiologyISBN: 9785794169850
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TOC.tableOfContents
- Ch. 1 - Evolution, the Themes of Biology, and Scientific Inquiry8
- Ch. 2 - The Chemical Context of Life8
- Ch. 3 - Water and Life6
- Ch. 4 - Carbon and the Molecular Diversity of Life9
- Ch. 5 - The Structure and Function of Large Biological Molecules9
- Ch. 6 - A Tour of the Cell6
- Ch. 7 - Membrane Structure and Function6
- Ch. 8 - An Introduction to Metabolism6
- Ch. 9 - Cellular Respiration and Fermentation10
- Ch. 10 - Photosynthesis7
- Ch. 11 - Cell Communication7
- Ch. 12 - The Cell Cycle11
- Ch. 13 - Meiosis and Sexual Life Cycles10
- Ch. 14 - Mendel and the Gene Idea14
- Ch, 15 - The Chromosomal Basis of Inheritance6
- Ch. 16 - The Molecular Basis of Inheritance9
- Ch. 17 - Gene Expression: From Gene to Protein9
- Ch. 18 - Regulation of Gene Expression10
- Ch. 19 - Viruses6
- Ch. 20 - DNA Tools and Biotechnology7
- Ch. 21 - Genomes and Their Evolution8
- Ch. 22 - Descent with Modification: A Darwininan View of Life6
- Ch. 23 - The Evolution of Populations5
- Ch. 24 - The Origin of Species6
- Ch. 25 - The History of Life on Earth8
- Ch. 26 - Phylogeny and the Tree of Life8
- Ch. 27 - Bacteria and Archaea6
- Ch. 28 - Protists7
- Ch. 29 - Plant Diversity I: How Plants Colonized Land7
- Ch. 30 - Plant Diversity II: The Evolution of Seed Plants6
- Ch. 31 - Fungi5
- Ch. 32 - An Overview of Animal Diversity4
- Ch. 33 - An introduction to Invertebrates6
- Ch. 34 - The Origin and Evolution of Vertebrates7
- Ch. 35 - Vascular Plant Structure, Growth, and Development10
- Ch. 36 - Resource Acquisition and Transport in Vascular Plants8
- Ch. 37 - Soil and Plant Nutrition11
- Ch. 38 - Angiosperm Reproduction and Biotechnology8
- Ch. 39 - Plant Responses to Internal and External Signals8
- Ch. 40 - Basic Principles of Animal Form and Function8
- Ch. 41 - Animal Nutrition7
- Ch. 42 - Circulation and Gas Exchange7
- Ch. 43 - The Immune System5
- Ch. 44 - Osmoregulation and Excretion6
- Ch. 45 - Hormones and the Endocrine System8
- Ch. 46 - Animal Reproduction9
- Ch. 47 - Animal Development8
- Ch. 48 - Neurons, Synapses, and Signaling6
- Ch. 49 - Nervous Systems8
- Ch. 50 - Sensory and Motor Mechanisms5
- Ch. 51 Animal Behavior6
- Ch. 52 - An Introduction to Ecology and the Biosphere7
- Ch. 53 - Population Ecology9
- Ch. 54 - Community Ecology9
- Ch. 55 - Ecosystems and Restoration Ecology8
- Ch. 56 - Conservation Biology and Global Change6
Capitolo 20, Problema 5
Which of the following is true of cDNA produced using human brain tissue as the starting material?
a. The procedure to make it requires amplification by the polymerase chain reaction.
b. It is produced from pre-mRNA using reverse transcriptase.
c. It can be labeled and used as a probe to detect genes expressed in the brain.
d. It includes the introns of the pre-mRNA.
Guida verificata passo dopo passo1
Understand that cDNA, or complementary DNA, is synthesized from an mRNA template using the enzyme reverse transcriptase. This process involves converting RNA sequences into DNA sequences.
Recognize that cDNA does not contain introns. Introns are non-coding regions found in pre-mRNA, but they are removed during mRNA processing before reverse transcription occurs.
Consider the role of cDNA in gene expression studies. cDNA can be labeled and used as a probe to detect specific genes that are actively expressed in a particular tissue, such as the brain.
Note that the polymerase chain reaction (PCR) is not required for the initial synthesis of cDNA. PCR is a technique used to amplify DNA sequences, but cDNA synthesis itself does not involve amplification.
Evaluate the options given: (A) is incorrect because PCR is not needed for cDNA synthesis; (B) is correct because cDNA is produced from mRNA using reverse transcriptase; (C) is correct because cDNA can be used as a probe; (D) is incorrect because cDNA does not include introns.
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cDNA
Complementary DNA (cDNA) is synthesized from an mRNA template using the enzyme reverse transcriptase. This process involves converting the single-stranded mRNA into double-stranded DNA, which represents only the expressed genes without introns, as introns are removed during mRNA processing.
Reverse Transcriptase
Reverse transcriptase is an enzyme used to generate cDNA from an RNA template. It catalyzes the synthesis of DNA from RNA, allowing researchers to study gene expression by creating a stable DNA copy of the mRNA present in cells, which can be further analyzed or used in various applications.
Video consigliato:
04:16
1) Initiation of Transcription
Gene Expression Analysis
Gene expression analysis involves studying the patterns of genes that are actively transcribed into mRNA in a cell or tissue. cDNA can be labeled and used as a probe to detect specific genes expressed in tissues like the brain, helping researchers understand which genes are active under certain conditions or in specific tissues.
Video consigliato:
06:40Introduction to Regulation of Gene Expression
Pratica correlata
Domanda del libro di testo
Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of
a. Post-transcriptional processing.
b. Post-translational processing.
c. Nucleic acid hybridization.
d. Restriction fragment ligation.
1898
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Domanda del libro di testo
Plants are more readily manipulated by genetic engineering than are animals because
a. Plant genes do not contain introns.
b. More vectors are available for transferring recombinant DNA into plant cells.
c. A somatic plant cell can often give rise to a complete plant.
d. Plant cells have larger nuclei.
2112
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Domanda del libro di testo
A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?
a. SNP analysis
b. Polymerase chain reaction (PCR)
c. Electroporation
d. Gel electrophoresis
1712
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Domanda del libro di testo
Which of the following tools of DNA technology is incorrectly paired with its use?
a. Electrophoresis — Separation of DNA fragments
b. DNA ligase — Cutting DNA, creating sticky ends of restriction fragments
c. DNA polymerase — Polymerase chain reaction to amplify sections of DNA
d. Reverse transcriptase — Production of cDNA from mRNA
1174
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