Question

Using mutants 2 and 3 from Problem 19, following mixed infection on E. coli B, progeny viruses were plated in a series of dilutions on both E. coli B and K12 with the following results.
Table showing progeny virus counts: 2 plaques on E. coli B at 10^-5 dilution, 5 plaques on E. coli K12 at 10^-1 dilution.

Table showing progeny virus counts: 2 plaques on E. coli B at 10^-5 dilution, 5 plaques on E. coli K12 at 10^-1 dilution.

Another mutation, 6, was tested in relation to mutations 1 through 5 from Problems 18–20. In initial testing, mutant 6 complemented mutants 2 and 3. In recombination testing with 1, 4, and 5, mutant 6 yielded recombinants with 1 and 5, but not with 4. What can you conclude about mutation 6?

Accepted Answer

Understand the problem: The question involves analyzing complementation and recombination data to determine the relationship of mutation 6 with other mutations. Complementation tests reveal whether two mutations affect the same gene, while recombination tests indicate whether mutations are located on the same or different regions of the genome. Analyze the complementation data: Mutation 6 complements mutants 2 and 3. This means that mutation 6 is in a different gene than the genes affected by mutations 2 and 3. Complementation occurs when two mutations are in different genes, allowing the functional products of each gene to compensate for the other's defect. Analyze the recombination data: Mutation 6 yields recombinants with mutations 1 and 5, but not with mutation 4. Recombination occurs when two mutations are on different regions of the same gene or on different genes. The absence of recombinants with mutation 4 suggests that mutation 6 and mutation 4 are very closely linked or in the same location within the same gene. Combine the complementation and recombination results: Since mutation 6 complements mutations 2 and 3, it is in a different gene than those mutations. The recombination data further suggests that mutation 6 is in the same gene as mutation 4 but in a different region than mutations 1 and 5. Conclude the relationship of mutation 6: Based on the data, mutation 6 is in the same gene as mutation 4 but in a different gene than mutations 2 and 3. The recombination with mutations 1 and 5 indicates that mutation 6 is in a different region of the genome compared to those mutations.

Risposta video verificata per un problema simile:

Concetti chiave

Complementation

Recombination

Mutant Analysis