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For a eukaryotic gene whose transcription requires the activity of an enhancer sequence, explain how proteins bound at the enhancer interact with RNA pol II and transcription factors bound at the promoter.
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Ch. 8 - Molecular Biology of Transcription and RNA Processing
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172
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TOC.tableOfContents
- Ch. 1 - The Molecular Basis of Heredity, Variation, and Evolution64
- Ch. 2 - Transmission Genetics144
- Ch. 3 - Cell Division and Chromosome Heredity81
- Ch. 4 - Gene Interaction87
- Ch. 5 - Genetic Linkage and Mapping in Eukaryotes103
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages73
- Ch. 7 - DNA Structure and Replication71
- Ch. 8 - Molecular Biology of Transcription and RNA Processing79
- Ch. 9 - The Molecular Biology of Translation110
- Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization100
- Ch. 11 - Gene Mutation, DNA Repair, and Homologous Recombination86
- Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage90
- Ch. 13 - Regulation of Gene Expression in Eukaryotes38
- Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics72
- Ch. 15 - Recombinant DNA Technology and Its Applications69
- Ch. 16 - Genomics: Genetics from a Whole-Genome Perspective49
- Ch. 17 - Organelle Inheritance and the Evolution of Organelle Genomes27
- Ch. 18 - Developmental Genetics43
- Ch. 19 - Genetic Analysis of Quantitative Traits66
- Ch. 20 - Population Genetics and Evolution at the Population, Species, and Molecular Levels105
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Non è quello che usi tu?Cambia libro di testo
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Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblema 14
Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblema 14Capitolo 8, Problema 14
Three genes identified in the diagram as A, B, and C are transcribed from a region of DNA. The 5'-to-3' transcription of genes A and C elongates mRNA in the right-to-left direction, and transcription of gene B elongates mRNA in the left-to-right direction. For each gene, identify the coding strand by designating it as an 'upper strand' or 'lower strand' in the diagram.
Guida verificata passo dopo passo1
Understand the concept of transcription: During transcription, the RNA polymerase reads the template strand of DNA in the 3'-to-5' direction to synthesize mRNA in the 5'-to-3' direction. The coding strand is the DNA strand that has the same sequence as the mRNA (except that thymine is replaced by uracil in RNA).
Analyze the direction of transcription for each gene: For genes A and C, transcription elongates mRNA in the right-to-left direction. For gene B, transcription elongates mRNA in the left-to-right direction.
Determine the template strand for each gene: The template strand is complementary to the mRNA strand. For genes A and C, since transcription occurs right-to-left, the lower strand must be the template strand. For gene B, since transcription occurs left-to-right, the upper strand must be the template strand.
Identify the coding strand for each gene: The coding strand is the strand opposite to the template strand. For genes A and C, the upper strand is the coding strand because the lower strand is the template strand. For gene B, the lower strand is the coding strand because the upper strand is the template strand.
Summarize the results: For gene A, the coding strand is the upper strand. For gene B, the coding strand is the lower strand. For gene C, the coding strand is the upper strand.
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Transcription Directionality
Transcription directionality refers to the orientation in which RNA polymerase synthesizes mRNA from a DNA template. In eukaryotic cells, transcription occurs in the 5'-to-3' direction, meaning that nucleotides are added to the 3' end of the growing mRNA strand. This directionality is crucial for understanding which DNA strand serves as the template and how the resulting mRNA aligns with the coding sequence.
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09:16
Eukaryotic Transcription
Coding and Template Strands
In the context of DNA transcription, the coding strand (also known as the sense strand) has the same sequence as the mRNA (except for uracil replacing thymine), while the template strand (antisense strand) is complementary to the mRNA. Identifying which strand is the coding strand is essential for determining the correct sequence of the mRNA produced during transcription, as it dictates the protein-coding potential of the gene.
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11:43The Genetic Code
Gene Orientation
Gene orientation refers to the arrangement of genes on a DNA molecule, which can be oriented in either direction. This orientation affects how transcription occurs, as genes can be transcribed from either the upper or lower strand of the DNA. Understanding gene orientation is vital for correctly identifying which strand corresponds to each gene's transcription direction, as seen in the question regarding genes A, B, and C.
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09:09Mapping Genes
Pratica correlata
Domanda del libro di testo
The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.
Are intron regions single stranded or double stranded? Why?
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Domanda del libro di testo
Draw a bacterial promoter and label its consensus sequences. How does this promoter differ from a eukaryotic promoter transcribed:
By RNA polymerase II?
By RNA polymerase I?
By RNA polymerase III?
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Domanda del libro di testo
Describe the difference between introns and exons.
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Domanda del libro di testo
The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.
Illustrate the R-loop structure that would be seen with electron microscopy.
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Domanda del libro di testo
The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.
Label the introns.
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