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Ch. 9 - Controlling Microbial Growth in the Environment
Bauman - Microbiology with Diseases by Taxonomy 6th Edition
Bauman6th EditionMicrobiology with Diseases by TaxonomyISBN: 9780134832302Non è quello che usi tu?Cambia libro di testo
Tutti i libri di testoBauman 6th EditionCh. 9 - Controlling Microbial Growth in the EnvironmentProblema 8
Capitolo 9, Problema 8

Which of the following items functions most like an autoclave?
a. Boiling pan
b. Incinerator
c. Microwave oven
d. Pressure cooker

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1
Understand the function of an autoclave: it sterilizes equipment and media by using steam under high pressure, which raises the boiling point of water and kills microorganisms effectively.
Analyze each option by comparing its mechanism to that of an autoclave:
a. Boiling pan: uses boiling water at atmospheric pressure, which reaches only 100°C, less effective for sterilization compared to an autoclave.
b. Incinerator: uses very high heat to burn materials, which is a different sterilization method and not similar to an autoclave's steam and pressure process.
c. Microwave oven: uses microwave radiation to heat food, but does not use steam under pressure, so it functions differently from an autoclave.
d. Pressure cooker: uses steam under pressure to cook food, increasing the boiling point of water similar to an autoclave, making it the closest functional equivalent.

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Autoclave Function and Mechanism

An autoclave sterilizes equipment by using pressurized steam at high temperatures, typically 121°C under 15 psi pressure, to kill all microorganisms including spores. This combination of heat and pressure ensures effective sterilization beyond simple boiling.
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Functional Groups

Boiling vs. Pressurized Steam Sterilization

Boiling water reaches 100°C at atmospheric pressure, which can kill many microbes but is less effective against spores. Pressurized steam, as in an autoclave, raises the boiling point and penetrates materials better, providing more reliable sterilization.
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Moist Heat

Pressure Cooker as a Sterilization Tool

A pressure cooker operates by trapping steam under pressure, increasing the temperature above 100°C, similar to an autoclave. This makes it functionally comparable for sterilization purposes, often used in labs or kitchens to sterilize or cook efficiently.
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High Pressure Processing
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