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Ch. 13 - Viruses, Viroids, and Prions
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Capitolo 9, Problema 3

A virus with RNA-dependent RNA polymerase
a. synthesizes DNA from an RNA template.
b. synthesizes double-stranded RNA from an RNA template.
c. synthesizes double-stranded RNA from a DNA template.
d. transcribes mRNA from DNA.
e. none of the above

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1
Step 1: Understand the function of RNA-dependent RNA polymerase (RdRp). This enzyme synthesizes RNA from an RNA template, which is a key feature of certain RNA viruses.
Step 2: Analyze each option in the context of RdRp activity: (a) synthesizes DNA from RNA template - this describes reverse transcriptase, not RdRp.
Step 3: Consider option (b) synthesizes double-stranded RNA from an RNA template - this matches the activity of RdRp, which can create complementary RNA strands to form double-stranded RNA.
Step 4: Evaluate options (c) and (d): (c) synthesizes double-stranded RNA from DNA template - this is incorrect because RdRp uses RNA, not DNA, as a template; (d) transcribes mRNA from DNA - this is the role of DNA-dependent RNA polymerase, not RdRp.
Step 5: Conclude that the correct description of RNA-dependent RNA polymerase activity is option (b), synthesizing double-stranded RNA from an RNA template.

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RNA-dependent RNA polymerase (RdRp)

RNA-dependent RNA polymerase is an enzyme used by RNA viruses to replicate their RNA genomes. It synthesizes a complementary RNA strand using an RNA template, enabling the production of new viral RNA. This enzyme is essential for RNA virus replication but is not involved in DNA synthesis.
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1) RNA Processing

RNA virus replication mechanisms

RNA viruses replicate their genomes by synthesizing RNA from RNA templates, often producing double-stranded RNA intermediates. This process differs from DNA viruses, which use DNA-dependent enzymes. Understanding this mechanism helps distinguish how viral RNA is copied and how mRNA is produced for protein synthesis.
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Animal Viruses: RNA Virus Synthesis & Replication

Transcription and reverse transcription

Transcription typically refers to synthesizing RNA from a DNA template, while reverse transcription involves making DNA from an RNA template. RNA-dependent RNA polymerase does not perform these functions; instead, it synthesizes RNA from RNA, distinguishing it from DNA-dependent RNA polymerase and reverse transcriptase.
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Initiation of Transcription