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Ch. 9 - Biotechnology & DNA Technology
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Capitolo 5, Problema 8

A population of cells carrying a desired plasmid.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector

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1
Understand the key terms provided: 'antisense', 'clone', 'library', 'Southern blot', and 'vector'. Each term has a specific meaning in molecular biology and microbiology.
Focus on the phrase 'A population of cells carrying a desired plasmid.' This implies multiple cells that all contain the same plasmid, which is a circular DNA molecule used in cloning.
Recall that a 'clone' in microbiology refers to a group of genetically identical cells derived from a single ancestor cell, often carrying the same plasmid.
Match the statement to the term that best fits the description of identical cells carrying the same plasmid, which is 'clone'.
Confirm that other options do not fit: 'antisense' relates to RNA sequences, 'library' is a collection of DNA fragments, 'Southern blot' is a DNA detection method, and 'vector' is the DNA molecule used to carry foreign DNA but not the population of cells itself.

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Clone

A clone refers to a population of genetically identical cells derived from a single ancestor cell, often carrying the same plasmid or DNA sequence. In molecular biology, cloning is used to amplify a specific DNA fragment within host cells.
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Introduction to DNA Cloning

Plasmid

A plasmid is a small, circular DNA molecule independent of chromosomal DNA, commonly found in bacteria. It can replicate autonomously and is often used as a vector to carry foreign genes into host cells for cloning or expression.
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F Plasmids

Vector

A vector is a DNA molecule, such as a plasmid or virus, used to transfer genetic material into a host cell. Vectors contain necessary elements for replication and selection, enabling the propagation and expression of inserted genes.
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Cloning with Recombinant DNA