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Ch. 9 - Biotechnology & DNA Technology
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Tutti i libri di testoTortora 14th EditionCh. 9 - Biotechnology & DNA TechnologyProblema 7
Capitolo 5, Problema 7

Pieces of human DNA stored in yeast cells.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector

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1
Understand the key terms provided: antisense, clone, library, Southern blot, and vector, as they relate to molecular biology and microbiology techniques.
Recall that a 'library' in molecular biology refers to a collection of DNA fragments stored in host cells, such as bacteria or yeast, representing the genome or a subset of DNA sequences.
Recognize that 'pieces of human DNA stored in yeast cells' describes a DNA library maintained within yeast, which is a common host for cloning large DNA fragments.
Match the description 'Pieces of human DNA stored in yeast cells' to the term 'library' because it best fits the concept of a collection of DNA fragments stored in a host organism.
Confirm that other options like 'clone' refer to identical copies of DNA, 'vector' is a DNA molecule used to carry foreign DNA into a host, 'antisense' relates to RNA sequences complementary to mRNA, and 'Southern blot' is a technique for detecting specific DNA sequences.

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Clone

A clone refers to a population of cells or DNA molecules derived from a single ancestor, containing identical genetic material. In molecular biology, cloning involves creating copies of specific DNA fragments, often by inserting them into host cells like yeast or bacteria for replication.
Video consigliato:
02:25
Introduction to DNA Cloning

Vector

A vector is a DNA molecule used as a vehicle to transfer foreign genetic material into a host cell. Common vectors include plasmids and viruses, which facilitate the replication and expression of inserted DNA fragments within host organisms such as yeast.
Video consigliato:
03:41
Cloning with Recombinant DNA

Library

A DNA library is a collection of cloned DNA fragments that represent the entire genome or a subset of an organism's DNA. These fragments are stored within host cells, like yeast, allowing researchers to access and study specific genes or sequences.
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You have a small gene that you want replicated by PCR. You add fluorescent dye-labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands will fluoresce?

a. 0%

b. 12.5%

c. 50%

d. 87.5%

e. 100%

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Describe an rDNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, cDNA, RNA polymerase, reverse transcriptase.

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A population of cells carrying a desired plasmid.

a. Antisense

b. Clone

c. Library

d. Southern blot

e. Vector

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You are attempting to insert a gene for saltwater tolerance into a plant by using the Ti plasmid. In addition to the desired gene, you add a gene for tetracycline resistance (tet) to the plasmid. What is the purpose of the tet gene?

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Identify and mark each of the following in making cDNA: transcription, RNA processing, reverse transcription, DNA polymerase, cDNA.

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List at least two examples of the use of rDNA in medicine and in agriculture.

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