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Ch. 9 - Biotechnology & DNA Technology
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Tutti i libri di testoTortora 14th EditionCh. 9 - Biotechnology & DNA TechnologyProblema 4
Capitolo 5, Problema 4

The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?
a. Reverse transcriptase
b. Ribozyme
c. RNA polymerase
d. DNA polymerase

Guida verificata passo dopo passo
1
Understand the process of making complementary DNA (cDNA) from RNA. The first step involves synthesizing a single-stranded DNA from an RNA template.
Identify the enzyme responsible for this first step: reverse transcriptase, which synthesizes DNA from an RNA template.
After the single-stranded cDNA is made, the next step is to convert this single-stranded DNA into double-stranded DNA.
Determine which enzyme synthesizes the complementary DNA strand to form double-stranded DNA. This enzyme is DNA polymerase, which uses the single-stranded cDNA as a template.
Conclude that the second enzyme used in making cDNA is DNA polymerase, as it completes the synthesis of double-stranded cDNA.

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Reverse Transcriptase

Reverse transcriptase is an enzyme that synthesizes complementary DNA (cDNA) from an RNA template. It is the first enzyme used in cDNA synthesis, converting single-stranded RNA into single-stranded cDNA.
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Animal Viruses: Reverse-Transcribing Virus Synthesis & Replication

DNA Polymerase

DNA polymerase is an enzyme that synthesizes the complementary DNA strand by extending the single-stranded cDNA created by reverse transcriptase. It is the second enzyme used in cDNA synthesis to produce double-stranded cDNA.
Video consigliato:
04:01
DNA Polymerases

cDNA Synthesis Process

cDNA synthesis involves two main enzymatic steps: first, reverse transcriptase creates single-stranded cDNA from RNA; second, DNA polymerase synthesizes the complementary strand to form double-stranded cDNA, which can be used for cloning or analysis.
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04:19
1) RNA Processing
Pratica correlata
Domanda del libro di testo

Some commonly used restriction enzymes are listed in Table 9.1.

a. Indicate which enzymes produce sticky ends.

b. Of what value are sticky ends in making rDNA?

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Domanda del libro di testo

Which of the following is the fourth basic step to genetically modify a cell?

a. Transformation

b. Ligation

c. Plasmid cleavage

d. Restriction-enzyme digestion of gene

e. Isolation of gene

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Domanda del libro di testo

Suppose you want multiple copies of a gene you have synthesized. How would you obtain the necessary copies by cloning? By PCR?

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Domanda del libro di testo

Identify and mark each of the following in making cDNA: transcription, RNA processing, reverse transcription, DNA polymerase, cDNA.

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Domanda del libro di testo

The DNA probe, 3'-GGCTTA, will hybridize with which of the following?

a. 5'-CCGUUA

b. 5'-CCGAAT

c. 5'-GGCTTA

d. 3'-CCGAAT

e. 3'-GGCAAU

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Domanda del libro di testo

If you put a gene in a virus, the next step in genetic modification would be

a. insertion of a plasmid.

b. transformation.

c. transduction.

d. PCR.

e. Southern blotting.

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