Domanda del libro di testo
Pieces of human DNA stored in yeast cells.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector
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Ch. 9 - Biotechnology & DNA Technology
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398
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TOC.tableOfContents
- Ch. 1 - The Microbial World and You18
- Ch. 2 - Chemical Principles20
- Ch. 3 - Observing Microorganisms Through a Microscope18
- Ch. 7 - The Control of Microbial Growth20
- Ch. 9 - Biotechnology & DNA Technology20
- Ch. 10 - Classification of Microorganisms15
- Ch. 11 - The Prokaryotes: Domains Bacteria and Archaea14
- Ch. 12 - The Eukaryotes: Fungi, Algae, Protozoa, and Helminths17
- Ch. 13 - Viruses, Viroids, and Prions20
- Ch. 14 - Principles of Disease and Epidemiology21
- Ch. 15 - Microbial Mechanisms of Pathogenicity19
- Ch. 16 - Innate Immunity: Nonspecific Defenses of the Host20
- Ch. 17 - Adaptive Immunity: Specific Defenses of the Host20
- Ch. 18 - Practical Applications of Immunology20
- Ch. 19 - Disorders Associated with the Immune System19
- Ch. 20 - Antimicrobial Drugs19
- Ch. 21 - Microbial Diseases of the Skin and Eyes21
- Ch. 22 - Microbial Diseases of the Nervous System19
- Ch. 23 - Microbial Diseases of the Cardiovascular and Lymphatic Systems20
- Ch. 24 - Microbial Diseases of the Respiratory System20
- Ch. 25 - Microbial Diseases of the Digestive System20
- Ch. 26 - Microbial Diseases of the Urinary and Reproductive Systems19
- Ch. 27 - Environmental Microbiology20
- Ch. 28 - Applied and Industrial Microbiology19
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Capitolo 5, Problema 5
Identify and mark each of the following in making cDNA: transcription, RNA processing, reverse transcription, DNA polymerase, cDNA.
Guida verificata passo dopo passo1
Step 1: Understand the process of making cDNA (complementary DNA). It involves starting from an mRNA template and converting it back into DNA. The key stages include transcription, RNA processing, reverse transcription, and DNA synthesis.
Step 2: Identify 'transcription' as the process where DNA is used to make pre-mRNA. This occurs in the nucleus and involves RNA polymerase synthesizing RNA complementary to the DNA template strand.
Step 3: Mark 'RNA processing' as the modifications that pre-mRNA undergoes to become mature mRNA. This includes 5' capping, splicing out introns, and 3' polyadenylation, preparing the mRNA for export to the cytoplasm.
Step 4: Locate 'reverse transcription' as the step where the enzyme reverse transcriptase synthesizes cDNA from the mature mRNA template. This is the key step that converts RNA back into DNA.
Step 5: Identify 'DNA polymerase' as the enzyme that may be used to synthesize the second strand of DNA, creating double-stranded cDNA from the single-stranded cDNA produced by reverse transcriptase.
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Transcription
Transcription is the process where RNA is synthesized from a DNA template by RNA polymerase. It produces a primary RNA transcript that contains both exons and introns before any processing occurs.
Video consigliato:
04:16
Initiation of Transcription
RNA Processing
RNA processing modifies the primary RNA transcript into mature mRNA by splicing out introns, adding a 5' cap, and a 3' poly-A tail. This processed mRNA is then ready for translation or reverse transcription.
Video consigliato:
04:191) RNA Processing
Reverse Transcription and cDNA Synthesis
Reverse transcription uses reverse transcriptase to synthesize complementary DNA (cDNA) from processed mRNA. DNA polymerase can then be used to create double-stranded cDNA, which is essential for cloning or PCR applications.
Video consigliato:
04:28Animal Viruses: Reverse-Transcribing Virus Synthesis & Replication
Pratica correlata
Domanda del libro di testo
You have a small gene that you want replicated by PCR. You add fluorescent dye-labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands will fluoresce?
a. 0%
b. 12.5%
c. 50%
d. 87.5%
e. 100%
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Domanda del libro di testo
The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?
a. Reverse transcriptase
b. Ribozyme
c. RNA polymerase
d. DNA polymerase
2033
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Domanda del libro di testo
Describe an rDNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, cDNA, RNA polymerase, reverse transcriptase.
1314
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Domanda del libro di testo
Suppose you want multiple copies of a gene you have synthesized. How would you obtain the necessary copies by cloning? By PCR?
1499
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Domanda del libro di testo
If you put a gene in a virus, the next step in genetic modification would be
a. insertion of a plasmid.
b. transformation.
c. transduction.
d. PCR.
e. Southern blotting.
1435
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