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Ch. 9 - Biotechnology & DNA Technology
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Tutti i libri di testoTortora 14th EditionCh. 9 - Biotechnology & DNA TechnologyProblema 5
Capitolo 5, Problema 5

If you put a gene in a virus, the next step in genetic modification would be
a. insertion of a plasmid.
b. transformation.
c. transduction.
d. PCR.
e. Southern blotting.

Guida verificata passo dopo passo
1
Step 1: Understand the context of the problem, which involves genetic modification using a virus as a vector to carry a gene.
Step 2: Recall that after inserting a gene into a virus, the next step is to introduce this virus into a host cell to deliver the gene.
Step 3: Identify the process by which viruses transfer genetic material into bacterial cells, which is known as transduction.
Step 4: Review the other options: insertion of a plasmid involves plasmids, transformation is uptake of naked DNA, PCR is amplification of DNA, and Southern blotting is a detection method, none of which directly follow gene insertion into a virus.
Step 5: Conclude that the correct next step after putting a gene into a virus is the process of transduction, where the virus transfers the gene into the target cells.

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Genetic Modification Using Viruses

Viruses can be used as vectors to deliver foreign genes into host cells, a process central to genetic modification. This involves inserting a gene into the viral genome, which then infects the target cell, introducing the new genetic material.
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How to Use the Genetic Code

Transduction

Transduction is the process by which viruses transfer genetic material from one bacterium to another. It is a natural mechanism exploited in genetic engineering to introduce new genes into cells using viral vectors.
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Transduction

Transformation vs. Transduction

Transformation involves uptake of naked DNA by cells, while transduction uses viruses to transfer DNA. Understanding the difference helps identify the correct step after inserting a gene into a virus, which is transduction, not transformation.
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Transduction
Pratica correlata
Domanda del libro di testo

You have a small gene that you want replicated by PCR. You add fluorescent dye-labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands will fluoresce?

a. 0%

b. 12.5%

c. 50%

d. 87.5%

e. 100%

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Which of the following is the fourth basic step to genetically modify a cell?

a. Transformation

b. Ligation

c. Plasmid cleavage

d. Restriction-enzyme digestion of gene

e. Isolation of gene

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The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?

a. Reverse transcriptase

b. Ribozyme

c. RNA polymerase

d. DNA polymerase

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Describe an rDNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, cDNA, RNA polymerase, reverse transcriptase.

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Suppose you want multiple copies of a gene you have synthesized. How would you obtain the necessary copies by cloning? By PCR?

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Identify and mark each of the following in making cDNA: transcription, RNA processing, reverse transcription, DNA polymerase, cDNA.

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