Question 1

Which of the following best describes the primary function of an enzyme in a biological reaction?

Accepted Answer

To lower the activation energy required for the reaction.

Question 2

Which class of enzyme catalyzes the transfer of a phosphoryl group ($$ -PO_3^{2-} ) $$between substrates?

Accepted Answer

Kinases

Question 3

A reaction catalyzed by alcohol dehydrogenase is shown below:

$$\mathrm{CH_3CH_2OH + NAD^+ \longrightarrow CH_3CHO + NADH + H^+}$$

Which class of enzyme is responsible for this reaction?

Accepted Answer

Oxidoreductase

Question 4

Which of the following statements about enzyme specificity is correct?

Accepted Answer

Highly specific enzymes catalyze one type of reaction for a single substrate.

Question 5

Which subclass of hydrolases is responsible for breaking peptide bonds in proteins?

Accepted Answer

Proteases

Question 6

Which effect describes how enzymes lower the activation energy by inducing a strain on the bonds of the substrate?

Accepted Answer

Energy effect

Question 7

Which enzyme class catalyzes the rearrangement of atoms within a molecule to form an isomer?

Accepted Answer

Isomerases

Question 8

Which class of enzyme requires ATP hydrolysis to provide energy for the reaction to occur?

Accepted Answer

Ligases

Question 9

A patient is diagnosed with a genetic disorder that prevents the production of coenzymes. Which type of enzyme activity would be most directly affected?

Accepted Answer

Oxidoreductase activity

Question 10

Which model best explains how substrate binding triggers a conformational change in the enzyme?

Accepted Answer

Induced fit model

Question 11

Which of the following statements about enzyme activity is true?

Accepted Answer

Enzymes can be modified during a reaction but return to their original state after the reaction.

Question 12

A scientist increases the substrate concentration in an enzyme-catalyzed reaction while keeping enzyme concentration constant. What will happen to the reaction rate?

Accepted Answer

It will increase and then level off at a maximum rate.

Question 13

Which of the following best describes the effect of temperature on enzyme activity in humans?

Accepted Answer

Enzyme activity is highest at around 37°C and decreases at higher or lower temperatures.

Question 14

Which of the following scenarios would most likely result in a decrease in enzyme activity?

Accepted Answer

Raising the temperature above the enzyme's optimum.

Question 15

Which of the following is true regarding the relationship between enzyme concentration and reaction rate, assuming excess substrate is present?

Accepted Answer

The reaction rate increases linearly with enzyme concentration.

Question 16

Which of the following best explains why the human form of trypsin would not be active in a thermophile organism that lives at 75°C?

Accepted Answer

Trypsin denatures at temperatures much higher than 37°C.

Question 17

Which of the following is NOT a function of a cofactor in enzyme catalysis?

Accepted Answer

Serving as the main protein structure of the enzyme.

Question 18

Which of the following best describes the role of a holoenzyme?

Accepted Answer

It is the catalytically active enzyme with its cofactor or coenzyme bound.

Question 19

Which of the following reactions is catalyzed by a ligase?

Accepted Answer

$$\mathrm{CO_2$ + CH_3COCOO^- + ATP \longrightarrow C_4H_4O_5$$^{2-}$$ + ADP + $$HPO_4$$^{2-} + H^+}$$

Enzyme Classification, Mechanisms, and Regulation: Study Notes for GOB Chemistry

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