Q: Which formula is used to estimate the actual size of an object viewed under the microscope?

$$\text{Actual Size} = \frac{\text{Drawing Size}}{\text{Magnification}}$$

Q: A student measures the field of view under low power as 5 mm. If the total magnification is increased from 40x to 100x, what is the new field of view?

$$\text{New FOV} = \frac{5 \text{ mm} \times 40}{100} = 2 \text{ mm}$$

Q: A human cheek cell is approximately $$62.5 \ \mu m in $$diameter. If you observe it under 400x total magnification, what is the apparent size of the cell in your drawing if you draw it as 2.5 cm?

$$\text{Magnification} = \frac{2.5 \text{ cm}}{0.0625 \text{ mm}} = 400 \times$$

Q: Which formula would you use to convert 2.5 mm to micrometers ($$\mu m$$)?

$$2.5 \text{ mm} \times 1000 = 2500 \ \mu m$$

Question 1

Which part of the microscope is responsible for adjusting the amount of light that reaches the specimen?

Accepted Answer

Iris diaphragm

Question 2

If you are using a 10x ocular lens and a 40x objective lens, what is the total magnification?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

What is the correct sequence for focusing a specimen under the microscope?

Accepted Answer

Start with the lowest power objective, use coarse focus, then fine focus

Question 4

Which formula is used to estimate the actual size of an object viewed under the microscope?

Accepted Answer

$$	ext{Actual Size} = \frac{	ext{Drawing Size}}{	ext{Magnification}}$$

Question 5

When switching from a low power to a high power objective, what happens to the field of view?

Accepted Answer

It decreases

Question 6

A student measures the field of view under low power as 5 mm. If the total magnification is increased from 40x to 100x, what is the new field of view?

Accepted Answer

$$	ext{New FOV} = \frac{5 	ext{ mm} 	imes 40}{100} = 2 	ext{ mm}$$

Question 7

Which of the following best describes the function of the condenser on a microscope?

Accepted Answer

It focuses light onto the specimen

Question 8

If the working distance decreases as you switch to a higher power objective, what is the most likely reason?

Accepted Answer

The objective lens is longer and closer to the specimen

Question 9

A human cheek cell is approximately $$62.5 \ \mu m in $$diameter. If you observe it under 400x total magnification, what is the apparent size of the cell in your drawing if you draw it as 2.5 cm?

Accepted Answer

$$	ext{Magnification} = \frac{2.5 	ext{ cm}}{0.0625 	ext{ mm}} = 400 	imes$$

Question 10

Which of the following is NOT a correct safety or care rule for using a microscope?

Accepted Answer

Use coarse focus knob with high power objective

Question 11

What is the function of the coarse focus adjustment knob?

Accepted Answer

To move the stage rapidly for initial focusing

Question 12

Which part of the microscope should you use to move the slide smoothly in small increments while viewing a specimen?

Accepted Answer

Stage adjustment knob

Question 13

If you switch from a 10x objective to a 40x objective, what happens to the brightness of the field of view?

Accepted Answer

It decreases

Question 14

Which formula would you use to convert 2.5 mm to micrometers ($$\mu m$$)?

Accepted Answer

$$2.5 	ext{ mm} 	imes 1000 = 2500 \ \mu m$$

Question 15

A student observes three colored threads on a slide. Which thread is on top if it comes into focus first as the stage is raised?

Accepted Answer

The thread farthest from the slide

Question 16

Which of the following best describes the function of the fine focus adjustment knob?

Accepted Answer

It is used for precise focusing, especially under high power

Question 17

What is the correct formula to estimate the field of view at high power if you know the field of view at low power?

Accepted Answer

$$	ext{FOV}_{	ext{high}} = \frac{	ext{FOV}_{	ext{low}} 	imes 	ext{Total Mag}_{	ext{low}}}{	ext{Total Mag}_{	ext{high}}}$$

Question 18

Which of the following is the best definition of 'resolution' in microscopy?

Accepted Answer

The ability to distinguish two close objects as separate entities

Question 19

When preparing a wet mount, what is the correct sequence of steps?

Accepted Answer

Place the specimen, add liquid, apply the coverslip at an angle, blot excess liquid

Microscopy: Structure, Function, and Use in Anatomy & Physiology

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