Q: If the diameter of the low-power field is 2 mm at 50x total magnification, what is the diameter of the high-power field at 100x total magnification?

$$\frac{2 \text{ mm} \times 50}{100} = 1 \text{ mm}$$

Question 1

Which part of the microscope, as shown in the image, is responsible for supporting the slide while it is being viewed?

Accepted Answer

Stage

Question 2

If you are using a 10x ocular lens and a 40x objective lens, what is the total magnification achieved?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

Which of the following best describes the function of the condenser in a compound microscope?

Accepted Answer

It concentrates light on the specimen.

Question 4

What is the correct procedure for cleaning the lenses of a microscope?

Accepted Answer

With special lens paper and cleaner

Question 5

A microscope is described as 'parfocal.' What does this mean?

Accepted Answer

The slide should be in focus at higher magnifications once it is properly focused at lower magnifications.

Question 6

If the diameter of the low-power field is 2 mm at 50x total magnification, what is the diameter of the high-power field at 100x total magnification?

Accepted Answer

$$\frac{2 	ext{ mm} 	imes 50}{100} = 1 	ext{ mm}$$

Question 7

Which adjustment knob should only be used with the lowest-power objective lens?

Accepted Answer

Coarse adjustment knob

Question 8

Why is it necessary to center your object before switching to a higher power objective?

Accepted Answer

The field of view becomes smaller at higher magnification, so the object may move out of view.

Question 9

Which of the following statements about working distance is correct?

Accepted Answer

Working distance decreases as magnification increases.

Question 10

What is the main reason for using a coverslip when preparing a wet mount?

Accepted Answer

To prevent soiling or damaging the objective lens

Question 11

If you move the slide to the left on the stage, in which direction does the image appear to move when viewed through the microscope?

Accepted Answer

To the right

Question 12

Which part of the microscope regulates the amount of light passing through the condenser?

Accepted Answer

Iris diaphragm lever

Question 13

What is the resolving power of a typical compound microscope under ideal conditions?

Accepted Answer

$$0.2 \ \mu m$$

Question 14

Which of the following best describes 'depth of field' in microscopy?

Accepted Answer

The thickness of the specimen that is clearly in focus at one time

Question 15

When switching from low power to high power, what should you do to the light intensity and why?

Accepted Answer

Increase the light intensity because less light enters the objective at higher magnification

Question 16

A student is using the high-power objective lens but the working distance is about 1 cm. What is the most likely explanation?

Accepted Answer

The student is not actually seeing the specimen because the lens is too far from the slide.

Question 17

Which of the following is the correct formula for calculating the unknown field diameter (Field B) given a known field diameter (Field A) and their respective total magnifications?

Accepted Answer

$$	ext{Diameter of field B} = \frac{	ext{Diameter of field A} 	imes 	ext{Total magnification of field A}}{	ext{Total magnification of field B}}$$

Question 18

Which of the following is NOT a correct step in the proper transport of a microscope?

Accepted Answer

Swing the instrument during transport.

Question 19

Why should you never use the coarse adjustment knob with the high-power or oil immersion objective lenses?

Accepted Answer

It could drive the objective lens through the slide, breaking the slide and possibly damaging the lens.

Question 20

When preparing a wet mount, why is it important to lower the coverslip at a 45° angle?

Accepted Answer

To avoid trapping large air bubbles under the coverslip

The Compound Microscope: Structure, Function, and Use

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