how-can-the-inhibition-constant-for-the-free-enzyme-ki-and-the-enzyme-substrate-

Question

How can the inhibition constant for the free enzyme (KI) and the enzyme-substrate complex (K'I) be used to determine the preferred binding site of an inhibitor?

Accepted Answer

By comparing KI and K'I values, the site with the lower inhibition constant indicates the preferred binding site.

Answer

By comparing KI and K'I values, the site with the higher inhibition constant indicates the preferred binding site.

Answer

The preferred binding site is determined by the substrate concentration, not KI or K'I values.

Answer

The preferred binding site is determined by the enzyme's reaction velocity, not KI or K'I values.