How does the inhibition constant for the free enzyme (KI) differ from the inhibition constant for the enzyme-substrate complex (K'I)?

KI measures the binding affinity of the enzyme-substrate complex for the inhibitor, while K'I measures the binding affinity of the free enzyme for the inhibitor.

KI and K'I both measure the binding affinity of the enzyme-substrate complex for the inhibitor.

KI and K'I both measure the binding affinity of the free enzyme for the inhibitor.

KI measures the binding affinity of the free enzyme for the inhibitor, while K'I measures the binding affinity of the enzyme-substrate complex for the inhibitor.