design-an-experiment-to-test-the-role-of-the-srp-in-co-translational-import-of-p

Question

Design an experiment to test the role of the SRP in co-translational import of proteins into the ER.

Accepted Answer

Use a mutant cell line lacking SRP and compare protein localization to wild-type cells.

Answer

Assess the formation of disulfide bonds in proteins in cells with and without SRP.

Answer

Measure the rate of GTP hydrolysis in cells with and without SRP.

Answer

Compare the glycosylation patterns of proteins in cells with and without SRP.