Which mechanism best describes how bacitracin interferes with bacterial cell wall synthesis?

It irreversibly inhibits the active site of penicillin-binding proteins responsible for peptide cross-linking of peptidoglycan, forming a stable covalent complex that stops transpeptidation.

It enzymatically cleaves the glycosidic bonds of existing peptidoglycan polymers rendering the cell wall soluble and resulting in immediate lysis of the bacterium due to osmotic instability.

It blocks the dephosphorylation and recycling of the lipid carrier bactoprenol, preventing transport of peptidoglycan precursors across the membrane.

It intercalates into DNA, preventing replication and thereby indirectly halting cell wall synthesis because new wall components cannot be produced when replication is inhibited.