You could identify an unknown bacterium by all of the following except
a. hybridizing a DNA probe from a known bacterium with the unknown’s DNA.
b. making a fatty acid profile of the unknown.
c. specific antiserum agglutinating the unknown.
d. ribosomal RNA sequencing.
e. percentage of guanine + cytosine.

검증된 단계별 안내

Step 1: Understand the methods listed for identifying bacteria. Each method uses different biological or chemical characteristics to distinguish bacterial species.

Step 2: Review DNA probe hybridization (option a), which involves using a known DNA sequence to find complementary sequences in the unknown bacterium's DNA, a common molecular identification technique.

Step 3: Consider fatty acid profiling (option b), which analyzes the types and proportions of fatty acids in bacterial membranes; this method is used for bacterial identification based on biochemical composition.

Step 4: Examine the use of specific antiserum agglutination (option c), where antibodies bind to bacterial surface antigens causing clumping; this is a serological method for identifying bacteria.

Step 5: Evaluate ribosomal RNA sequencing (option d) and percentage of guanine + cytosine content (option e), both molecular methods that provide genetic information useful for bacterial classification and identification. Determine which method is least likely or not typically used for bacterial identification.

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주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

DNA Probe Hybridization

DNA probe hybridization involves using a labeled DNA fragment from a known organism to detect complementary sequences in an unknown bacterium's DNA. This method helps identify bacteria by confirming genetic similarity or presence of specific genes.

Fatty Acid Profiling

Fatty acid profiling analyzes the types and proportions of fatty acids in bacterial cell membranes. Since fatty acid composition varies among species, this biochemical method aids in bacterial identification and classification.

Guanine + Cytosine Content

The percentage of guanine plus cytosine (G+C content) in bacterial DNA reflects the overall nucleotide composition. While useful for broad classification, G+C content alone is insufficient for precise identification of unknown bacteria.