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What would occur if a normal dNTP were substituted for a ddNTP during the PCR amplification step in dideoxy sequencing?
A researcher is analyzing the results of a Sanger sequencing gel electrophoresis. Which of the following best describes how to read the DNA sequence from the gel?
In a laboratory exercise involving Sanger sequencing, a DNA segment with the sequence 5'-GCTAAGCTTAG-3' is used. Which of the following sequences correctly represents the termination sequence when ddTTP is incorporated?
In a laboratory experiment, a student is analyzing a DNA sample through PCR amplification followed by gel electrophoresis. The student observes four distinct bands on the gel corresponding to sequences containing only adenine (A), thymine (T), cytosine (C), and guanine (G), respectively. If the sequence obtained from the gel is 5'-CGTA-3', what is the sequence of the complementary DNA strand?