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Bacteria for lab exam + steps of gram staining

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  • what are the steps of gram staining?

    1) Crystal violet on slide - 1 minute

    WASH WITH WATER

    2) Grams Iodine on slide - 1 minute

    WASH WITH WATER

    3) Wash with alcohol until it runs clear

    WASH OFF WITH WATER

    4) Safranin on slide - 45 seconds

    BLOT DRY WITH BIBULOUS PAPER

  • Streptococcus Pyogenes

    Gram +

    Chain of cocci

    "Strep" = chain

    Treated with penicillin

  • Mycobacterium tuberculosis

    Gram +

    rod shaped, thick capsule

    airborne pathogen

  • Staphylococcus aureus (S. aureus)

    G+

    cluster of cocci

    looks like grape clusters under microscope

    "Staph" = cluster

    has large, round golden-yellow colonies on blood agar

    catalase + = able to break down hydrogen peroxide to H20 and O2

  • Penicillium notatum

    mold/fungus

    produces penicillin

    penicillin works against G+ bacteria

    osmotolerant = can thrive in environments with high osmotic pressure

  • how do you write Streptococcus Pyogenes

    Streptococcus pyogenes

  • how do you write Mycobacterium tuberculosis?

    Mycobacterium tuberculosis

  • how do you write Staphylococcus aureus?

    Staphylococcus aurueus

  • how do you wirte Penicillium nonatum?

    Penicillium nonatum

  • what does Streptococcus pyogenes look like under the microscope?

    spherical to ovoid-shaped cocci arranged in pairs or long chains

  • what does Mycobacterium tuberculosis look like under the microscope?

    bacillus - small, straight, or slightly curved rod-shaped bacterium

    in clusters or stacked together in parallel bundles (cigarettes)

  • what does Staphyloccus aureus look like under the microscope?

    tiny cocci (round shapes) arranged in tightly packed clusters (like grapes)

  • what does Penicillium notatum look like under the microscope?

    tube-like filaments (filamentous)

    resembles a tiny broom or paintbrush