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Microbiology Exam 1 Study Guide

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  • Microbiology


    It is the study of microorganisms and their relevance to human health, the environment, and industry.

  • Major groups of microorganisms

    Bacteria, Archaea, Fungi, Protozoa, Algae, and Viruses.

  • Difference between cellular and acellular life

    Cellular life consists of cells (bacteria, archaea, fungi, protozoa, algae), while acellular life includes viruses, viroids, and prions which lack cellular structure.

  • Why viruses are not considered living organisms

    Viruses lack cellular structure and cannot reproduce or carry out metabolism without a host cell, making them obligate intracellular parasites.

  • Spontaneous generation vs. biogenesis

    Spontaneous generation is the disproven idea that life arises from nonliving matter; biogenesis states that life arises from existing life.

  • Pasteur's experiment significance

    Pasteur's experiment refuted spontaneous generation by showing that sterilized broth remained free of microbes unless exposed to contaminated air.

  • Contributions of Koch

    Koch developed postulates to link specific microbes to specific diseases, advancing germ theory.

  • How organisms are named and classified (Taxonomy)

    Organisms are named using binomial nomenclature (Genus species) and classified based on shared characteristics.

  • Miller’s Experiment main goal and outcome

    To simulate early Earth conditions and show organic molecules like amino acids could form from inorganic compounds.

  • Dehydration synthesis reaction

    A chemical reaction that joins monomers into polymers by removing water molecules.

  • Four major biological macromolecules

    Carbohydrates, Lipids, Proteins, and Nucleic acids.

  • Function of functional groups in biochemistry

    Functional groups determine the chemical properties and reactivity of organic molecules.

  • Cell theory key principles

    All living things are made of cells, cells are the basic unit of life, and all cells come from preexisting cells.

  • Differences between prokaryotic and eukaryotic cells

    Prokaryotes lack a nucleus and membrane-bound organelles; eukaryotes have both.

  • Endosymbiotic theory

    Explains the origin of eukaryotic cells from prokaryotic ancestors through symbiosis.

  • Gram-positive vs. Gram-negative cell walls

    Gram-positive have thick peptidoglycan layers; Gram-negative have thin layers plus an outer membrane.

  • Extremophiles

    Prokaryotes that thrive in extreme environments like high temperature, acidity, or salinity.

  • Phototrophs vs. Chemotrophs

    Phototrophs obtain energy from light; chemotrophs obtain energy from chemical compounds.

  • Autotrophs vs. Heterotrophs

    Autotrophs fix carbon dioxide to make organic compounds; heterotrophs consume organic compounds.

  • Normal microbiota vs. pathogens

    Normal microbiota are harmless or beneficial microbes living on/in the body; pathogens cause disease.

  • Major groups of eukaryotic microbes

    Protists, fungi, and helminths (e.g., platyhelminths).

  • Motility structures in eukaryotic microbes

    Flagella, cilia, and pseudopodia enable movement.

  • Differences between yeasts and molds

    Yeasts are unicellular fungi; molds are multicellular and produce toxins.

  • Viral structure components

    Capsid, genome (DNA or RNA), and sometimes an envelope.

  • Lytic vs. lysogenic viral cycles

    Lytic cycle destroys host cells by producing new virions; lysogenic cycle integrates viral DNA into host genome.

  • Why viruses are obligate intracellular parasites

    They require host cellular machinery to replicate and cannot reproduce independently.

  • Retrovirus characteristics

    Contain RNA genome and use reverse transcriptase to make DNA from RNA (e.g., HIV).

  • Methods to culture and detect viruses

    Hemagglutination, PCR, and ELISA (EIA) are common viral detection methods.

  • Viroids and prions

    Viroids are infectious ssRNA molecules; prions are misfolded infectious proteins.