Skip to main content
Back

Column Chromatography definitions

Control buttons has been changed to "navigation" mode.
1/14
  • Column Chromatography
    A protein purification method using a vertical tube packed with solid material and liquid to separate components by their attractions.
  • Protein Purification
    A process to isolate specific proteins from a mixture, often completed by separating them in a column with different affinities.
  • Stationary Phase
    An immobile solid inside a column that interacts with sample components, enabling their separation based on attraction.
  • Mobile Phase
    A liquid that flows through a column, carrying sample components and facilitating their separation by interacting with the stationary material.
  • Affinity
    The strength of attraction between sample components and the materials in a column, determining how they separate.
  • Elution
    The process where separated components exit the column and are collected, marking the completion of their separation.
  • Silica
    A common solid used as the immobile material in columns, providing a surface for sample interactions during separation.
  • Crude Extract
    The initial mixture obtained from cells, containing all cellular components before purification steps.
  • Sample Size
    The amount of material processed in purification, often reduced before using expensive separation techniques.
  • Charge
    A property of proteins that can be used to separate them in columns by their attraction to materials with opposite electrical characteristics.
  • Size
    A characteristic of molecules that allows their separation in columns based on how easily they move through the stationary material.
  • Polarity
    A molecular property influencing separation in columns, based on the attraction to materials with similar or different polar characteristics.
  • Tube Collection
    The method of gathering separated proteins at the column's base in individual containers for further analysis.
  • Separation Technique
    A method used to distinguish and isolate components from a mixture, often based on their physical or chemical properties.