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Edman Degradation Sequenator and Sequencing Data Analysis definitions

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  • Edman Degradation
    Automated method for sequencing proteins by removing and identifying amino acids from the N-terminal end.
  • Sequenator
    Instrument that automates reagent mixing, product separation, and data recording for protein sequencing.
  • PTH Amino Acid
    Final product of Edman degradation, used for identification in sequencing via chromatographic analysis.
  • High-Performance Liquid Chromatography
    Technique that separates and identifies PTH amino acids by comparing elution positions to known controls.
  • Chromatogram
    Graphical output displaying absorbance versus elution time, revealing peptide sequence order.
  • Elution Time
    Duration required for an amino acid to exit the chromatography column, used for identification.
  • Absorbance
    Measurement on the chromatogram's y-axis indicating the presence and quantity of amino acids.
  • N-terminal
    Starting end of a peptide sequence, where Edman degradation begins and sequencing is read left to right.
  • C-terminal
    Ending end of a peptide sequence, reached last in Edman degradation sequencing.
  • Mass Spectrometry
    Analytical technique that reads peptide sequences from right to left but struggles with isomer differentiation.
  • Isomer
    Molecule with identical mass and chemical formula as another, such as Leucine and Isoleucine.
  • Amino Acid Control
    Reference sample with known elution position used to identify unknown PTH amino acids in HPLC.
  • Peptide Sequence
    Order of amino acids in a protein, revealed by chromatogram peaks from N-terminal to C-terminal.
  • Nanogram
    Extremely small mass unit, indicating the sensitivity of detection in Edman degradation.
  • Picomole
    Tiny amount of substance, demonstrating the minimal quantity detectable by the sequenator.