Biochemistry

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5. Protein Techniques

Edman Degradation Sequenator and Sequencing Data Analysis

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Edman Degradation Sequenator and Sequencing Data Analysis

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Problem

Separately determine the peptide sequences indicated by the HPLC chromatogram & mass spectrum below.

A) What is the peptide sequence indicated by the HPLC chromatogram?

B) What is the peptide sequence indicated by the mass spectrum (assuming prominent peaks correspond to y ions)?