Skip to main content
Back

Native Gel Electrophoresis definitions

Control buttons has been changed to "navigation" mode.
1/15
  • Native Gel Electrophoresis
    Technique separating proteins by their inherent charge, shape, and size, preserving their original structure during migration.
  • Native PAGE
    Abbreviation for a method using polyacrylamide gels to analyze proteins in their unaltered form based on physical properties.
  • Polyacrylamide
    Organic compound forming the gel matrix, providing a stable environment for protein separation in electrophoresis.
  • Electric Field
    Force generated across the gel, driving charged proteins toward the electrode with opposite polarity.
  • Electrode
    Terminal in the gel apparatus, establishing positive and negative ends to direct protein movement.
  • Sample Well
    Indentation in the gel where protein samples are loaded before separation begins.
  • Band
    Visible region on the gel after staining, indicating the position and quantity of a specific protein.
  • Mass
    Physical property influencing protein migration speed; larger values result in slower movement through the gel.
  • Charge
    Intrinsic property determining the direction and extent of protein migration in the electric field.
  • Shape
    Structural feature affecting how proteins navigate the gel matrix, impacting migration even with identical mass and charge.
  • Subunit
    Individual component of a protein, remaining associated during native PAGE, preserving overall structure.
  • Staining
    Process applied after electrophoresis to visualize protein bands, enabling analysis of separation results.
  • Migration
    Movement of proteins through the gel, determined by their mass, charge, and shape.
  • Net Charge
    Total electrical property of a protein, dictating its interaction with the electric field and influencing migration distance.
  • Gel Matrix
    Supportive structure composed of polyacrylamide, facilitating protein separation based on physical characteristics.