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Native Gel Electrophoresis quiz

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  • What does native gel electrophoresis (native PAGE) separate proteins based on?
    Native PAGE separates proteins based on their native charge, shape, and size.
  • What is the role of the electric field in native PAGE?
    The electric field causes charged proteins to migrate through the gel towards the electrode with the opposite charge.
  • How do negatively charged proteins move in a native PAGE setup?
    Negatively charged proteins move towards the positive electrode in the gel.
  • How do positively charged proteins behave in native PAGE?
    Positively charged proteins move towards the negative electrode in the gel.
  • What happens to proteins with no net charge in native PAGE?
    Proteins with no net charge do not migrate through the gel.
  • How does protein size affect migration in native PAGE?
    Larger proteins migrate more slowly through the gel, while smaller proteins migrate faster.
  • Why is polyacrylamide used in native PAGE?
    Polyacrylamide forms the gel matrix that proteins move through during electrophoresis.
  • What does the thickness of a protein band on a stained gel indicate?
    The thickness of a band indicates the quantity of that protein present.
  • Can proteins with the same mass and shape migrate differently in native PAGE? Why or why not?
    Yes, because differences in their net charge can cause them to migrate differently.
  • How can protein shape affect migration in native PAGE?
    Proteins with the same mass and charge but different shapes can migrate differently due to shape influencing movement through the gel.
  • What are the three main factors that influence protein migration in native PAGE?
    Mass, charge, and shape are the three main factors.
  • Why does native PAGE retain protein subunits together?
    Because proteins retain their native structure, including subunit associations, during native PAGE.
  • What is a limitation of native PAGE compared to SDS PAGE?
    Native PAGE is more difficult to analyze because migration depends on three factors, not just one.
  • Why might you choose native PAGE for protein analysis?
    Native PAGE is useful when you want to analyze proteins in their native, functional forms.
  • What is the main difference between native PAGE and SDS PAGE in terms of protein migration?
    In native PAGE, migration depends on mass, charge, and shape, while in SDS PAGE, migration depends mainly on mass.