Skip to main content
Back

Strategy for Ordering Cleaved Fragments definitions

Control buttons has been changed to "navigation" mode.
1/15
  • Peptide Fragment
    A segment resulting from protein cleavage, used to reconstruct the original sequence by overlapping with other segments.
  • Trypsin
    A peptidase that cleaves at the C-terminal side of lysine and arginine residues, generating distinct peptide fragments.
  • Chymotrypsin
    A peptidase preferring aromatic residues for cleavage, producing fragments useful for sequence reconstruction.
  • Pepsin
    A peptidase that cleaves N-terminal peptide bonds of specific residues, aiding in identification of terminal fragments.
  • Amino Acid Residue
    A building block of peptides, whose position and identity determine cleavage sites and fragment overlaps.
  • C-terminal Fragment
    A segment at the end of a peptide lacking terminal residues recognized by the cleavage reagent, crucial for sequence assembly.
  • N-terminal Fragment
    A segment at the start of a peptide without terminal residues recognized by the cleavage reagent, key for sequence orientation.
  • Cleavage Reagent
    A chemical or enzyme used to break specific peptide bonds, generating fragments for sequencing analysis.
  • Sequence Reconstruction
    The process of assembling the original peptide order by overlapping fragments from different cleavage methods.
  • Overlapping Fragments
    The technique of aligning peptide segments with shared residues to reveal the complete sequence.
  • Terminal Residue
    An amino acid at the end of a fragment, whose recognition or absence by a reagent helps identify terminal fragments.
  • Aromatic Residue
    A type of amino acid, such as phenylalanine, tyrosine, or tryptophan, often targeted by chymotrypsin for cleavage.
  • Backup Plan
    A strategy involving starting with the longest fragment when standard overlapping fails to reconstruct the sequence.
  • Protein Composition
    Information about the number and types of amino acids in a peptide, used as clues in fragment ordering.
  • Peptide Bond
    A chemical linkage between amino acids, whose cleavage by reagents produces fragments for sequencing.