Question 1

Which part of the microscope is responsible for adjusting the amount of light that reaches the specimen?

Accepted Answer

Diaphragm

Question 2

If you are using a 10x eyepiece and a 40x objective lens, what is the total magnification?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

What is the function of the coarse focus knob on a light microscope?

Accepted Answer

To move the stage up and down for rough focusing

Question 4

Which term describes the ability of a microscope to keep a specimen in focus when switching between objective lenses?

Accepted Answer

Parfocal

Question 5

What is the primary purpose of the condenser in a light microscope?

Accepted Answer

To focus light onto the specimen

Question 6

Which part of the microscope should you use for fine adjustments when viewing a specimen under high magnification?

Accepted Answer

Fine focus knob

Question 7

A student observes a blurry image under the microscope at 100x magnification. Which adjustment should be made first?

Accepted Answer

Use the coarse focus knob to bring the specimen into rough focus

Question 8

What is the function of the mechanical stage knob on a microscope?

Accepted Answer

To move the slide horizontally and vertically

Question 9

Which part of the microscope is used to hold the slide securely in place?

Accepted Answer

Stage clips

Question 10

If the resolution of a microscope is defined as the minimum distance between two points that can still be distinguished as separate, which formula best represents this concept?

Accepted Answer

$$d = \frac{\lambda}{2NA}$$

Question 11

Why is it important to start focusing with the lowest power objective lens?

Accepted Answer

It allows for easier location of the specimen and prevents damage to the slide

Question 12

Which part of the microscope connects the base to the head and is used for carrying the instrument?

Accepted Answer

Arm

Question 13

A student wants to increase the brightness of the specimen. Which part should they adjust?

Accepted Answer

Light source

Question 14

What is the function of the nosepiece on a microscope?

Accepted Answer

To rotate and change the objective lenses

Question 15

Which term describes the clarity or sharpness of an image produced by a microscope?

Accepted Answer

Resolution

BIOL 120 Lab Final Study Guide: Key Concepts and Procedures

Study Guide - Practice Questions

Study Guide - Flashcards