Q: Which of the following is the correct formula for calculating the total magnification of a compound microscope?

$$\text{Total Magnification} = \text{Ocular lens} \times \text{Objective lens}$$

Question 1

Which of the following best describes the function of the coarse focus knob on a compound light microscope?

Accepted Answer

It moves the stage up and down rapidly to bring the specimen into general focus.

Question 2

If the ocular lens has a magnification of $$10	imes$$ and the low power objective lens is $$10	imes$$, what is the total magnification?

Accepted Answer

$$100	imes$$

Question 3

When switching from low power to high power objective, what happens to the field of view?

Accepted Answer

It gets smaller.

Question 4

Which part of the microscope is responsible for controlling the amount of light that reaches the specimen?

Accepted Answer

Diaphragm

Question 5

A student observes a specimen under the scanning objective lens ($$4	imes) $$with a field of view of $$4.5\ 	ext{mm}. $$What is the field of view under the low power objective ($$10	imes$$)? Use the formula: $$	ext{FOV}_	ext{low} = 	ext{FOV}_	ext{scan} 	imes \frac{	ext{Mag}_	ext{scan}}{	ext{Mag}_	ext{low}}$$

Accepted Answer

$$1.8\ 	ext{mm}$$

Question 6

Which of the following is the correct sequence for focusing a specimen under high power?

Accepted Answer

Start with the scanning objective, use the coarse focus knob, then switch to high power and use the fine focus knob.

Question 7

What is the main advantage of using a compound light microscope over a simple magnifying glass?

Accepted Answer

It provides higher magnification and better resolution due to multiple lenses.

Question 8

If you move the slide to the right on the stage, in which direction does the image appear to move when viewed through the microscope?

Accepted Answer

To the left

Question 9

Which lens is closest to the specimen when the microscope is in use?

Accepted Answer

Objective lens

Question 10

A student wants to estimate the size of a cell under high power. The field of view under high power is $$0.45\ 	ext{mm}$$ and the cell occupies about one-third of the field. What is the approximate size of the cell?

Accepted Answer

$$0.15\ 	ext{mm}$$

Question 11

Which of the following best explains the difference between resolving power and magnification power of a lens?

Accepted Answer

Resolving power is the ability to distinguish two points as separate, while magnification power is how much larger an object appears.

Question 12

Which step is NOT part of the proper cleanup procedure for a compound light microscope?

Accepted Answer

Leave the slide on the stage for the next user.

Question 13

Why is it important to only use lens paper to clean microscope lenses?

Accepted Answer

Other materials may scratch the lens and reduce image quality.

Question 14

Which of the following is the correct formula for calculating the total magnification of a compound microscope?

Accepted Answer

$$	ext{Total Magnification} = 	ext{Ocular lens} 	imes 	ext{Objective lens}$$

Question 15

A student prepares a wet mount slide of pond water. Which of the following steps is essential for making a proper wet mount?

Accepted Answer

Place a drop of water on the slide, add the specimen, and gently lower a coverslip at an angle.

Question 16

If the field of view under the scanning lens ($$4	imes) is$$ $$4.5\ 	ext{mm}$$, what is the field of view under the high power lens ($$40	imes$$)? Use the formula: $$	ext{FOV}_	ext{high} = 	ext{FOV}_	ext{scan} 	imes \frac{	ext{Mag}_	ext{scan}}{	ext{Mag}_	ext{high}}$$

Accepted Answer

$$0.45\ 	ext{mm}$$

Question 17

Which part of the microscope should you use to move the slide left and right on the stage?

Accepted Answer

Stage traverse knobs

Question 18

When examining a colored thread slide, why do some threads appear in focus while others do not?

Accepted Answer

Threads are at different depths, and only one plane can be in sharp focus at a time.

Question 19

Which of the following best describes the function of the diaphragm on a compound microscope?

Accepted Answer

It controls the amount of light passing through the specimen.

Question 20

Why is it important to always start focusing with the scanning or low power objective lens?

Accepted Answer

It reduces the risk of damaging the slide or lens.

Introduction to Microscopy and the Compound Light Microscope

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