Which of the following statements about the oxidation of pyruvate to acetyl CoA is/are correct? (Select all that apply.)
I. NAD⁺ is reduced to NADH during the oxidation of pyruvate.
II. The reaction occurs in the cytoplasm of the cell.
III. Coenzyme A (CoA) is required to form acetyl CoA.
IV. Pyruvate oxidation directly produces ATP.

• A. I only
• B. IV only
• C. I and III
• D. I, III, and IV

Answer: (C)