after-identifying-disulfide-linked-peptides-using-diagonal-electrophoresis-what-

Question

After identifying disulfide-linked peptides using diagonal electrophoresis, what is the next step to determine the positions of disulfide bonds?

Accepted Answer

Isolate the peptides and subject them to sequencing.

Answer

Perform a second round of electrophoresis.

Answer

Treat the peptides with a reducing agent.

Answer

Use mass spectrometry to analyze the peptides.