what-role-do-ampholytes-play-in-establishing-a-stable-ph-gradient-in-isoelectric

Question

What role do ampholytes play in establishing a stable pH gradient in isoelectric focusing?

Accepted Answer

They distribute along the gel to create a stable pH gradient.

Answer

They increase the gel's conductivity.

Answer

They act as a buffer to maintain a constant pH.

Answer

They denature proteins to facilitate separation.