in-a-2d-electrophoresis-experiment-two-proteins-with-identical-isoelectric-point

Question

In a 2D electrophoresis experiment, two proteins with identical isoelectric points are separated. What allows for this separation?

Accepted Answer

The SDS PAGE step separates them based on their different molecular weights.

Answer

The proteins are separated based on their hydrophobicity.

Answer

The isoelectric focusing step separates them based on their different charges.

Answer

The proteins are separated based on their tertiary structure.