Domanda del libro di testo
Kornberg showed that nucleotides are added to the 3' end of each growing DNA strand. In what way does an exposed 3'-OH group participate in strand elongation?
926
views
Ch. 10 - DNA Replication
Klug10th EditionEssentials of GeneticsISBN: 9780135588789
Non è quello che usi tu?Cambia libro di testoScegli un libro di testo diverso
TOC.tableOfContents
- Ch. 1 - Introduction to Genetics16
- Ch. 2 - Mitosis and Meiosis28
- Ch. 3 - Mendelian Genetics37
- Ch. 4 - Modification of Mendelian Ratios53
- Ch. 5 - Sex Determination and Sex Chromosomes32
- Ch. 6 - Chromosome Mutations: Variation in Number and Arrangement37
- Ch. 7 - Linkage and Chromosome Mapping in Eukaryotes36
- Ch. 8 - Genetic Analysis and Mapping in Bacteria and Bacteriophages24
- Ch. 9 - DNA Structure and Analysis38
- Ch. 10 - DNA Replication29
- Ch. 11 - Chromosome Structure and DNA Sequence Organization22
- Ch. 12 - The Genetic Code and Transcription36
- Ch. 13 - Translation and Proteins27
- Ch. 14 - Gene Mutation, DNA Repair, and Transposition34
- Ch. 15 - Regulation of Gene Expression in Bacteria22
- Ch. 16 - Regulation of Gene Expression in Eukaryotes37
- Ch. 17 - Recombinant DNA Technology27
- Ch. 18 - Genomics, Bioinformatics, and Proteomics30
- Ch. 19 - The Genetics of Cancer21
- Ch. 20 - Quantitative Genetics and Multifactorial Traits37
- Ch. 21 - Population and Evolutionary Genetics45
Klug10th EditionEssentials of GeneticsISBN: 9780135588789Non è quello che usi tu?Cambia libro di testo
Capitolo 10, Problema 7
What are the requirements for in vitro synthesis of DNA under the direction of DNA polymerase I?
Guida verificata passo dopo passo1
Understand that DNA polymerase I requires a template strand of DNA to guide the synthesis of the new DNA strand, so the first requirement is a single-stranded DNA template.
Recognize that DNA polymerase I cannot initiate DNA synthesis de novo; it needs a primer with a free 3'-OH group to add nucleotides, so a primer annealed to the template is necessary.
Provide the four deoxyribonucleotide triphosphates (dNTPs): dATP, dTTP, dGTP, and dCTP, which serve as the building blocks for the new DNA strand.
Include the DNA polymerase I enzyme itself, which catalyzes the polymerization reaction by adding nucleotides to the primer in the 5' to 3' direction.
Ensure the reaction mixture contains an appropriate buffer with Mg^{2+} ions, as these are essential cofactors for the enzymatic activity of DNA polymerase I.
Risposta video verificata per un problema simile:
Questa soluzione video è stata consigliata dai nostri tutor come utile per risolvere questo problema.
Durata del video:
1mConcetti chiave
Ecco i concetti essenziali che devi comprendere per rispondere correttamente alla domanda.
Role of DNA Polymerase I
DNA Polymerase I is an enzyme that synthesizes DNA by adding nucleotides to a pre-existing strand. It requires a template strand to guide the sequence and can also remove RNA primers through its exonuclease activity, making it essential for DNA replication and repair.
Video consigliato:
Percorso guidato
01:45
DNA Proofreading
Requirements for DNA Synthesis
In vitro DNA synthesis by DNA Polymerase I requires a single-stranded DNA template, a primer with a free 3'-OH group to initiate synthesis, all four deoxynucleotide triphosphates (dNTPs) as substrates, and appropriate buffer conditions including Mg2+ ions for enzyme activity.
Video consigliato:
Percorso guidato
02:43Translesion Synthesis
Primer-Template Structure
DNA Polymerase I cannot start DNA synthesis de novo; it needs a primer annealed to the template strand. The primer provides the free 3'-OH end necessary for the enzyme to add nucleotides, ensuring accurate and efficient DNA elongation.
Video consigliato:
Percorso guidato
03:49Ribosome Structure
Pratica correlata
Domanda del libro di testo
How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?
727
views
Domanda del libro di testo
Compare conservative, semiconservative, and dispersive modes of DNA replication.
1299
views
Domanda del libro di testo
Which characteristics of DNA polymerase I raised doubts that its in vivo function is the synthesis of DNA leading to complete replication?
855
views
Domanda del libro di testo
Describe the role of ¹⁵N in the Meselson–Stahl experiment.
918
views
Domanda del libro di testo
Predict the results of the experiment by Taylor, Woods, and Hughes if replication were
(a) conservative and
(b) dispersive.
1078
views