Domanda del libro di testo
Define plaque, lysogeny, and prophage.
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Ch. 8 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
Klug10th EditionEssentials of GeneticsISBN: 9780135588789
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TOC.tableOfContents
- Ch. 1 - Introduction to Genetics16
- Ch. 2 - Mitosis and Meiosis28
- Ch. 3 - Mendelian Genetics37
- Ch. 4 - Modification of Mendelian Ratios53
- Ch. 5 - Sex Determination and Sex Chromosomes32
- Ch. 6 - Chromosome Mutations: Variation in Number and Arrangement37
- Ch. 7 - Linkage and Chromosome Mapping in Eukaryotes36
- Ch. 8 - Genetic Analysis and Mapping in Bacteria and Bacteriophages24
- Ch. 9 - DNA Structure and Analysis38
- Ch. 10 - DNA Replication29
- Ch. 11 - Chromosome Structure and DNA Sequence Organization22
- Ch. 12 - The Genetic Code and Transcription36
- Ch. 13 - Translation and Proteins27
- Ch. 14 - Gene Mutation, DNA Repair, and Transposition34
- Ch. 15 - Regulation of Gene Expression in Bacteria22
- Ch. 16 - Regulation of Gene Expression in Eukaryotes37
- Ch. 17 - Recombinant DNA Technology27
- Ch. 18 - Genomics, Bioinformatics, and Proteomics30
- Ch. 19 - The Genetics of Cancer21
- Ch. 20 - Quantitative Genetics and Multifactorial Traits37
- Ch. 21 - Population and Evolutionary Genetics45
Klug10th EditionEssentials of GeneticsISBN: 9780135588789Non è quello che usi tu?Cambia libro di testo
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Klug 10th EditionCh. 8 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblema 13
Klug 10th EditionCh. 8 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblema 13Capitolo 8, Problema 13
In the plaque assay, exactly what makes up a single plaque?
Guida verificata passo dopo passo1
Understand that a plaque assay is a method used to quantify the number of virus particles capable of infecting cells in a sample.
Recognize that a single plaque represents a clear zone on a layer of host cells where the virus has infected and lysed the cells.
Know that each plaque originates from a single infectious virus particle, also called a plaque-forming unit (PFU).
Realize that the virus infects a host cell, replicates, and spreads to neighboring cells, causing cell death and forming the visible plaque.
Therefore, a single plaque is made up of a localized area of lysed host cells resulting from infection by one infectious virus particle.
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Plaque Assay
A plaque assay is a laboratory method used to quantify the number of infectious virus particles in a sample by infecting a layer of host cells. Each infectious virus particle infects a cell and spreads to neighboring cells, creating a clear zone called a plaque where cells have been destroyed.
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03:44
Plaques and Experiments
Plaque Formation
A single plaque forms when one infectious virus particle infects a host cell and replicates, causing cell lysis. The infection spreads locally to adjacent cells, resulting in a visible clear area or plaque, which represents the progeny of that original virus particle.
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03:44Plaques and Experiments
Relationship Between Plaques and Virus Particles
Each plaque corresponds to one infectious virus particle, also called a plaque-forming unit (PFU). Counting plaques allows estimation of the concentration of infectious viruses in the original sample, assuming each plaque arises from a single virus.
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03:44Plaques and Experiments
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Domanda del libro di testo
The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
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Domanda del libro di testo
A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted three times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 17 plaques. What is the initial density of bacteriophages in the original 1 mL?
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