The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

Write the DNA sequence of both alleles, including strand polarity.

Common baker's yeast (Saccharomyces cerevisiae) is normally grown at 37°C, but it will grow actively at temperatures down to approximately 25°C. A haploid culture of wild-type yeast is mutagenized with EMS. Cells from the mutagenized culture are spread on a complete-medium plate and grown at 25°C. Six colonies (1 to 6) are selected from the original complete-medium plate and transferred to two fresh complete-medium plates. The new complete plates (shown) are grown at 25°C and 37°C. Four replica plates are made onto minimal medium or minimal plus adenine from the 25°C complete-medium plate. The new plates are grown at either 25°C or 37°C and the growth results are shown.

Classify the nature of the mutations in colonies 1, 2, and 5.

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

Identify the template and nontemplate strands of DNA.

Common baker's yeast (Saccharomyces cerevisiae) is normally grown at 37°C, but it will grow actively at temperatures down to approximately 25°C. A haploid culture of wild-type yeast is mutagenized with EMS. Cells from the mutagenized culture are spread on a complete-medium plate and grown at 25°C. Six colonies (1 to 6) are selected from the original complete-medium plate and transferred to two fresh complete-medium plates. The new complete plates (shown) are grown at 25°C and 37°C. Four replica plates are made onto minimal medium or minimal plus adenine from the 25°C complete-medium plate. The new plates are grown at either 25°C or 37°C and the growth results are shown.

Which colonies are prototrophic and which are auxotrophic? What growth information is used to make these determinations?

Common baker's yeast (Saccharomyces cerevisiae) is normally grown at 37°C, but it will grow actively at temperatures down to approximately 25°C. A haploid culture of wild-type yeast is mutagenized with EMS. Cells from the mutagenized culture are spread on a complete-medium plate and grown at 25°C. Six colonies (1 to 6) are selected from the original complete-medium plate and transferred to two fresh complete-medium plates. The new complete plates (shown) are grown at 25°C and 37°C. Four replica plates are made onto minimal medium or minimal plus adenine from the 25°C complete-medium plate. The new plates are grown at either 25°C or 37°C and the growth results are shown.

What can you say about colony 4?

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

Determine the amino acid sequences translated from these mRNAs.

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

Write out the mRNA sequences encoded by each template strand, and underline the start codons.