Ligase catalyzes a reaction between the 5′ phosphate and the 3′ hydroxyl groups at the ends of DNA molecules. The enzyme calf intestinal phosphatase catalyzes the removal of the 5′5′ phosphate from DNA molecules. What would be the consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase?
Ch. 15 - Recombinant DNA Technology and Its Applications

15장, 문제 2b
The human genome is 3×10⁹ bp in length.
How would your initial answer change if you knew that the average GC content of the human genome was 40%?
검증된 단계별 안내1
Step 1: Understand the problem. The human genome is 3×10⁹ base pairs (bp) in length, and the average GC content is 40%. GC content refers to the percentage of guanine (G) and cytosine (C) bases in the genome. The remaining 60% would consist of adenine (A) and thymine (T) bases.
Step 2: Calculate the total number of GC base pairs. Multiply the total genome length (3×10⁹ bp) by the GC content percentage (40%). Use the formula: .
Step 3: Calculate the total number of AT base pairs. Subtract the number of GC base pairs from the total genome length. Use the formula: .
Step 4: If needed, determine the number of each individual base (G, C, A, T). Since GC pairs are equally distributed between G and C, divide the total GC base pairs by 2 to find the number of G and C bases. Similarly, divide the total AT base pairs by 2 to find the number of A and T bases.
Step 5: Summarize the results. The GC content changes the distribution of bases in the genome, affecting calculations related to base composition, melting temperature, and other genomic properties.

비슷한 문제에 대한 검증된 영상 답변:
이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.
영상 길이:
2m도움이 되었나요?
주요 개념
질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.
Human Genome Size
The human genome consists of approximately 3 billion base pairs (bp), which encodes the genetic information necessary for the development and functioning of a human being. Understanding the size of the genome is crucial for grasping the complexity of genetic information and its implications in genetics, genomics, and evolutionary biology.
추천 영상:
가이드 코스
Human Genome Composition
GC Content
GC content refers to the percentage of guanine (G) and cytosine (C) bases in a DNA molecule. In the human genome, an average GC content of 40% indicates that 40% of the base pairs are either G or C. This metric is important as it can influence the stability of the DNA molecule, gene expression, and the overall structure of the genome.
추천 영상:
가이드 코스
Genomics Overview
Implications of GC Content on Genome Function
The GC content can affect various genomic features, including mutation rates, recombination frequency, and the binding affinity of proteins to DNA. A higher GC content often correlates with increased stability of the DNA double helix, which can impact gene regulation and the evolution of genomic regions. Understanding these implications is essential for interpreting genetic data and its biological significance.
추천 영상:
가이드 코스
Functional Genomics
관련 실천
교과서 질문
432
views
교과서 질문
The human genome is 3×10⁹ bp in length.
How many fragments would be predicted to result from the complete digestion of the human genome with the following enzymes: Sau3A (˘GATC), BamHI (G˘GATCC), EcoRI (G˘AATTC), and NotI (GC˘GGCCGC)?
1481
views
교과서 질문
Compare and contrast the terms Paternity Index (PI) and Combined Paternity Index (CPI). How does each contribute to paternity determination?
545
views
교과서 질문
What is the statistical principle underlying genetic health risk assessment? Why are these assessments not predictive of disease occurrence?
432
views
교과서 질문
What is the exclusion principle? How is it used in forensic genetic analysis and in paternity determination?
678
views
교과서 질문
What is CODIS? Describe the four most important features of genetic markers used in this system.
772
views
