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Ch. 8 - Molecular Biology of Transcription and RNA Processing
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172당신이 사용하는 게 아니라요?교과서 변경
모든 교과서Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA Processing문제 30a
8장, 문제 30a

Genomic DNA from a mouse is isolated, fragmented, and denatured into single strands. It is then mixed with mRNA isolated from the cytoplasm of mouse cells. The image represents an electron micrograph result showing the hybridization of single-stranded DNA and mRNA.
Which nucleic acid is indicated by the 'a' pointer? Justify your answer. 

검증된 단계별 안내
1
Understand the context of the problem: The question involves hybridization of single-stranded DNA (ssDNA) and mRNA. Hybridization occurs when complementary nucleic acid strands bind to each other, forming a DNA-RNA hybrid.
Analyze the experimental setup: Genomic DNA is fragmented and denatured into single strands, while mRNA is isolated from the cytoplasm. The hybridization process allows mRNA to bind to complementary sequences in the ssDNA.
Interpret the electron micrograph: The 'a' pointer likely indicates a specific nucleic acid structure. In such experiments, regions of ssDNA that do not hybridize with mRNA remain as loops, while hybridized regions form double-stranded DNA-RNA hybrids.
Justify the identity of the nucleic acid: If the 'a' pointer is marking a loop, it represents single-stranded DNA (ssDNA) that does not have a complementary sequence in the mRNA. This occurs because introns (non-coding regions) in the genomic DNA are absent in the mRNA, which only contains exons (coding regions).
Conclude the reasoning: The 'a' pointer indicates single-stranded DNA (ssDNA) because it corresponds to intronic regions that are not transcribed into mRNA and therefore cannot hybridize with the mRNA.

비슷한 문제에 대한 검증된 영상 답변:

이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.
영상 길이:
2m
도움이 되었나요?

주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Hybridization

Hybridization refers to the process where complementary nucleic acid strands bind together to form a double-stranded structure. In this context, single-stranded DNA from the mouse genome can hybridize with mRNA, which is complementary to the DNA sequence that was transcribed. This process is crucial for understanding how genetic information is expressed and can be visualized in experiments like the one described.
추천 영상:
가이드 코스
03:08
Drosophila P Element

mRNA (Messenger RNA)

mRNA, or messenger RNA, is a type of RNA that carries genetic information from DNA to the ribosome, where proteins are synthesized. It is synthesized during transcription and serves as a template for translation. In the context of the question, the mRNA isolated from mouse cells is essential for understanding which specific sequences of DNA are being expressed in the cells.
추천 영상:
가이드 코스
05:43
RNA

Electron Micrography

Electron micrography is a technique that uses a beam of electrons to create high-resolution images of biological specimens. This method allows researchers to visualize the hybridization of nucleic acids at a molecular level. In the question, the electron micrograph provides visual evidence of the interaction between the single-stranded DNA and mRNA, helping to identify the nucleic acid indicated by the 'a' pointer.
추천 영상:
가이드 코스
04:29
Induced Mutations
관련 실천
교과서 질문

Genomic DNA from a mouse is isolated, fragmented, and denatured into single strands. It is then mixed with mRNA isolated from the cytoplasm of mouse cells. The image represents an electron micrograph result showing the hybridization of single-stranded DNA and mRNA.

Which nucleic acid is indicated by the 'b' pointer? Justify your answer. 

559
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교과서 질문

The DNA sequence below gives the first 12 base pairs of the transcribed region of a gene, and the template and nontemplate strands of DNA are identified. The transcription start is the thymine nucleotide at the end of the sequence given. Use the diagram to answer the list of questions. Make a copy of the diagram before you begin answering the questions, or have one group member diagram the answers for bacteria and another group member diagram the answers for eukaryotes. Nontemplate strand ___________TTGCTACGGTCA___________

Template strand ___________AACGATGCCAGT___________

Write the anticipated bacterial consensus sequence(s) in the approximate position(s) on the diagram.

515
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교과서 질문

Genomic DNA from a mouse is isolated, fragmented, and denatured into single strands. It is then mixed with mRNA isolated from the cytoplasm of mouse cells. The image represents an electron micrograph result showing the hybridization of single-stranded DNA and mRNA.

What term best identifies the nucleic acid region indicated by the 'c' pointer?

578
views
교과서 질문

The DNA sequence below gives the first 12 base pairs of the transcribed region of a gene, and the template and nontemplate strands of DNA are identified. The transcription start is the thymine nucleotide at the end of the sequence given. Use the diagram to answer the list of questions. Make a copy of the diagram before you begin answering the questions, or have one group member diagram the answers for bacteria and another group member diagram the answers for eukaryotes.

Nontemplate strand ___________ TTGCTACGGTCA___________

Template strand    ___________ AACGATGCCAGT___________

Assuming the sequence shown is part of a eukaryotic gene, what consensus sequence(s) would you expect to identify within about 100 base pairs of the start of transcription?

508
views
교과서 질문

The DNA sequence below gives the first 12 base pairs of the transcribed region of a gene, and the template and nontemplate strands of DNA are identified. The transcription start is the thymine nucleotide at the end of the sequence given. Use the diagram to answer the list of questions. Make a copy of the diagram before you begin answering the questions, or have one group member diagram the answers for bacteria and another group member diagram the answers for eukaryotes.

Nontemplate strand ___________ TTGCTACGGTCA___________

Template strand    ___________ AACGATGCCAGT___________

Write the anticipated eukaryotic consensus sequence(s) in the approximate position(s) on the diagram.

456
views
교과서 질문

Genomic DNA from a mouse is isolated, fragmented, and denatured into single strands. It is then mixed with mRNA isolated from the cytoplasm of mouse cells. The image represents an electron micrograph result showing the hybridization of single-stranded DNA and mRNA.

What term best identifies the nucleic acid region indicated by the 'd' pointer? 

592
views