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Immunoassay: ELISA
26. Applications of the Immune Response / Immunoassay: ELISA / Problem 4
Problem 4

A standard curve is prepared with standards: 0, 10, 20, 40, 80 ng/mL giving absorbances of 0.05, 0.15, 0.25, 0.45, 0.85 respectively. An unknown sample wells reads 0.33 absorbance. Using a linear fit, estimate the concentration of the unknown sample in ng/mL.