Which statement best defines a monoclonal antibody (MAB)?

Which of the following lists the standard sequential steps for hybridoma-based large-scale monoclonal antibody production?

If the selective HAT medium step is accidentally omitted after fusion of B cells and myeloma cells, what is the most likely consequence for establishing stable hybridoma clones?

You start with 1.0 × 10⁷ splenic B cells from an immunized mouse; 0.2% of these B cells are specific for the injected antigen. Fusion conditions yield hybridomas from 0.5% of the total B cells (i.e., 0.5% of B cells successfully fuse to a myeloma). Estimate the number of antigen-specific hybridomas you expect to obtain after fusion (before any screening losses). Show your calculation.

Compare total antibody yields over 10 days for two cultures: Culture A starts with 1.0 × 10⁶ primary B cells that do not divide and secrete 1.0 × 10⁴ molecules/cell/day for 5 days before dying; Culture B starts with 1.0 × 10⁵ hybridoma cells that double every 48 hours (2 days) and secrete 2.0 × 10⁴ molecules/cell/day during the entire 10-day period. Calculate total molecules produced by each culture over the 10 days and determine the fold difference (Culture B / Culture A). Show your calculation steps.