Which of the following lists the standard sequential steps for hybridoma-based large-scale monoclonal antibody production?

• A. Immunize mouse → isolate splenic B cells and culture myeloma cells → chemically fuse cells → culture on selective media → screen and isolate a single hybridoma clone → expand the clone for production.
• B. Isolate splenic B cells → fuse with patient T cells → grow on non-selective media → bulk expand multiple clones together to capture polyclonal representation.
• C. Culture myeloma cells → immediately inject myeloma into a patient → isolate antibodies from blood → perform selection plate assay → sequence antibodies.
• D. Directly harvest monoclonal antibodies from the mouse serum after immunization without any cell culture or fusion steps, which produces unlimited quantities of a single antibody.

Answer: (A)