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Prototrophs vs. Auxotrophs definitions

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  • Prototroph

    Original parent strain with no mutations, capable of synthesizing all required growth factors from other molecules.
  • Auxotroph

    Mutant strain with DNA mutations, unable to synthesize certain growth factors, requiring them from the environment.
  • Growth Factor

    Essential molecule needed for cell survival and growth, which some cells must obtain from their surroundings.
  • Mutation

    Change in DNA sequence that can alter a cell's ability to produce necessary molecules for survival.
  • Parent Strain

    Original, unmutated cell line used as a reference for comparing genetic changes.
  • His+

    Genetic notation indicating the ability to synthesize histidine without environmental supplementation.
  • His-

    Genetic notation indicating the inability to synthesize histidine, requiring it from the environment.
  • Histidine

    Amino acid used in protein synthesis, often serving as a model growth factor in genetic studies.
  • Plus Sign

    Symbol used to represent strains capable of synthesizing their own growth factors.
  • Minus Sign

    Symbol used to represent strains that require external growth factors due to mutations.
  • Functional Gene

    DNA segment without mutations, enabling synthesis of essential molecules for cell survival.
  • Mutant Gene

    Altered DNA segment that disrupts the synthesis of required cellular molecules.
  • Amino Acid

    Building block of proteins, some of which must be synthesized or acquired for cell growth.
  • Environment

    External surroundings providing necessary molecules for cells unable to synthesize them.
  • Synthesis

    Cellular process of constructing complex molecules from simpler ones, crucial for growth factor production.