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Fundamentals of Microbiology

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  • What are microorganisms?

    Microorganisms are organisms too small to be seen with the unaided eye, including bacteria, fungi, protozoa, microscopic algae, viruses, and prions.

  • What roles do microbes play in the environment?

    Microbes decompose organic waste, form the basis of aquatic food chains, incorporate nitrogen gas into organic compounds, and generate oxygen by photosynthesis.

  • What is the human microbiome?

    The microbiome is the community of microbes living stably on or in the human body, helping maintain health, preventing pathogen growth, and training the immune system.

  • How are microorganisms named scientifically?

    Scientific names use binomial nomenclature: genus (capitalized) and specific epithet (lowercase), italicized or underlined, often Latinized and descriptive or honoring a scientist.

  • What are the main types of microorganisms?

    Types include bacteria, archaea, fungi, protozoa, algae, viruses, and multicellular animal parasites.

  • What are key characteristics of bacteria?

    Bacteria are unicellular prokaryotes with peptidoglycan cell walls, reproduce by binary fission, and may move using flagella.

  • How do archaea differ from bacteria?

    Archaea are prokaryotes lacking peptidoglycan walls, often live in extreme environments, and are not known to cause human disease.

  • What distinguishes fungi from other microbes?

    Fungi are eukaryotes with chitin cell walls, absorb organic chemicals for energy, and include unicellular yeasts and multicellular molds.

  • What are protozoa and their features?

    Protozoa are eukaryotes that absorb or ingest organic chemicals, may be motile, and reproduce sexually or asexually.

  • What defines algae in microbiology?

    Algae are eukaryotes with cellulose cell walls, found in water and soil, using photosynthesis to produce oxygen and carbohydrates.

  • What are viruses and how do they replicate?

    Viruses are acellular, consist of DNA or RNA core with a protein coat, and replicate only inside living host cells.

  • What is spontaneous generation vs. biogenesis?

    Spontaneous generation is the disproven idea that life arises from nonliving matter; biogenesis states living cells arise only from preexisting cells.

  • Who demonstrated the theory of biogenesis?

    Louis Pasteur demonstrated that microorganisms come from the air, not mystical forces, using S-shaped flasks that kept microbes out but allowed air in.

  • What was Pasteur's contribution to fermentation?

    Pasteur showed microbes cause fermentation, converting sugar to alcohol anaerobically, and spoilage by bacteria turning alcohol to vinegar.

  • What is pasteurization?

    Pasteurization is heating beverages briefly at high temperature to kill harmful bacteria without evaporating alcohol.

  • What is Koch's postulate?

    Koch's postulates are experimental steps to prove a specific microbe causes a specific disease, established after discovering the anthrax bacterium.

  • What is vaccination and its origin?

    Vaccination, derived from Latin vacca (cow), involves inoculating with a related virus (e.g., cowpox) to provide immunity against diseases like smallpox.

  • What defines the second golden age of microbiology?

    Focus on treating microbial diseases with chemotherapy, including synthetic drugs and antibiotics produced by bacteria and fungi.

  • Who discovered the first antibiotic and what was it?

    Alexander Fleming discovered penicillin in 1928, produced by Penicillium fungus, which kills Staphylococcus aureus.

  • What are biofilms and their significance?

    Biofilms are microbial communities attached to surfaces, which can protect mucous membranes or cause infections and antibiotic resistance.

  • What factors contribute to emerging infectious diseases?

    Factors include microbial evolution (e.g., antibiotic resistance), modern transportation, and increased human exposure to new infectious agents.

  • What is the role of microbes in sewage treatment?

    Microbes convert organic waste in sewage into by-products like carbon dioxide and nitrates, helping recycle water safely.

  • How are microbes used in insect pest control?

    Microbes pathogenic to insects, like Bacillus thuringiensis, produce toxins fatal to pests but safe for animals and plants, reducing chemical pesticide use.

  • What is recombinant DNA technology?

    Recombinant DNA involves combining DNA from different sources to produce proteins, vaccines, or replace defective genes in gene therapy.

  • What is microbial ecology?

    Microbial ecology studies interactions between microbes and their environment, including nutrient cycling of carbon, nitrogen, sulfur, and phosphorus.

  • What is the significance of the microbiome in human health?

    The microbiome helps prevent pathogen growth, produces vitamins B and K, and supports immune system function.

  • What is antibiotic resistance and give an example?

    Antibiotic resistance occurs when microbes evolve to survive drugs; examples include MRSA and multidrug-resistant Mycobacterium tuberculosis.

  • What is the germ theory of disease?

    The germ theory states that specific microbes cause specific diseases, supported by work from Pasteur, Lister, and Koch.

  • What is the difference between normal and transient microbiota?

    Normal microbiota colonize the body indefinitely and support health, while transient microbiota colonize temporarily without permanent residence.