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Microbial Genetics - Microbiology

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  • What is microbial genetics?

    Microbial genetics is the study of how microbes inherit traits and how their genetic material is organized, replicated, and expressed.

  • What are the main types of genetic material in microbes?

    The main types are DNA and sometimes RNA, which carry the genetic information in microbes.

  • Define genotype and phenotype in microbes.

    Genotype is the genetic makeup of a microbe; phenotype is the observable traits resulting from the genotype.

  • What is a gene?

    A gene is a segment of DNA that codes for a functional product, usually a protein.

  • Describe the structure of bacterial DNA.

    Bacterial DNA is typically a single, circular chromosome located in the nucleoid region.

  • What is a plasmid?

    A plasmid is a small, circular DNA molecule separate from the chromosome that can replicate independently.

  • What role do plasmids play in microbes?

    Plasmids often carry genes for antibiotic resistance or other survival advantages.

  • Explain the process of DNA replication in microbes.

    DNA replication is semiconservative, producing two DNA molecules each with one original and one new strand.

  • What enzymes are involved in microbial DNA replication?

    Key enzymes include DNA helicase, DNA polymerase, and ligase.

  • Define transcription in microbial genetics.

    Transcription is the process of copying DNA into messenger RNA (mRNA).

  • What is translation?

    Translation is the synthesis of proteins from mRNA by ribosomes.

  • What is the central dogma of molecular biology?

    The flow of genetic information: DNA β†’ RNA β†’ Protein.

  • What is a mutation?

    A mutation is a change in the nucleotide sequence of DNA.

  • Name types of mutations in microbes.

    Types include point mutations, insertions, deletions, and frameshift mutations.

  • What is horizontal gene transfer?

    Horizontal gene transfer is the movement of genetic material between microbes, not by inheritance.

  • List the three main mechanisms of horizontal gene transfer.

    Transformation, transduction, and conjugation.

  • Describe transformation in microbes.

    Transformation is uptake of free DNA from the environment by a microbe.

  • What is transduction?

    Transduction is gene transfer mediated by bacteriophages (viruses that infect bacteria).

  • Explain conjugation.

    Conjugation is direct transfer of DNA between two bacterial cells via a pilus.

  • Why is microbial genetics important?

    It helps understand microbial functions, antibiotic resistance, and biotechnology applications.