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Microbiology: Key Concepts and History

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  • What are microbes?

    Microorganisms too small to be seen with the unaided eye, including bacteria, fungi, protozoa, microscopic algae, viruses, and prions.

  • Define microbiome and normal microbiota.

    Microbiome is the group of microbes living stably on/in the human body. Normal microbiota are the acquired microorganisms on or in a healthy human, which may be permanent or transient.

  • List some roles of microbes.

    Microbes can be pathogenic, cause food spoilage, decompose waste, fix nitrogen, produce oxygen, create chemicals like ethanol, and produce fermented foods and medicines.

  • What is scientific nomenclature in microbiology?

    A system established by Linnaeus using two names: genus (capitalized) and specific epithet (lowercase), both italicized or underlined.

  • Characteristics of bacteria

    Prokaryotes with peptidoglycan cell walls, unicellular, reproduce by binary fission, and may have flagella for movement.

  • Characteristics of archaea

    Prokaryotes lacking peptidoglycan, often living in extreme environments, and not known to cause human disease.

  • Characteristics of fungi

    Eukaryotes with chitin cell walls; yeasts are unicellular, molds and mushrooms are multicellular with hyphae.

  • Characteristics of protozoa

    Eukaryotes that absorb or ingest organic chemicals, may be motile by pseudopods, cilia, or flagella, and reproduce sexually or asexually.

  • Characteristics of algae

    Eukaryotes with cellulose cell walls, found in water and soil, use photosynthesis to produce oxygen and carbohydrates.

  • Characteristics of viruses

    Acellular entities with DNA or RNA core, protein coat, sometimes lipid envelope, and replicate only inside living host cells.

  • What are multicellular animal parasites?

    Eukaryotic multicellular animals, including parasitic flatworms and roundworms (helminths), with some microscopic life stages.

  • What are the three domains of life?

    Bacteria, Archaea, and Eukarya (which includes protists, fungi, plants, and animals).

  • What is the cell theory?

    All living things are composed of cells, as first reported by Robert Hooke in 1665.

  • Define spontaneous generation and biogenesis.

    Spontaneous generation is the idea that life arises from nonliving matter. Biogenesis states that living cells arise only from preexisting living cells.

  • How did Pasteur disprove spontaneous generation?

    Using S-shaped flasks, Pasteur showed that broth remained free of microbes unless exposed to air containing microorganisms.

  • What is fermentation and pasteurization?

    Fermentation is microbial conversion of sugar to alcohol without air. Pasteurization uses heat to kill harmful bacteria without evaporating alcohol.

  • What is the germ theory of disease?

    The theory that microbes cause disease, supported by discoveries from Pasteur, Lister, and Koch.

  • What are Koch's postulates?

    Experimental steps to prove that a specific microbe causes a specific disease.

  • What is vaccination?

    Inoculation with a less harmful virus (e.g., cowpox) to provide immunity against a related disease (e.g., smallpox), discovered by Edward Jenner.

  • What is chemotherapy in microbiology?

    Treatment of disease using chemicals, including synthetic drugs and antibiotics produced by bacteria and fungi.

  • Who discovered the first antibiotic and what was it?

    Alexander Fleming discovered penicillin in 1928, produced by the Penicillium fungus.

  • Define bacteriology, mycology, parasitology, immunology, and virology.

    Bacteriology: study of bacteria; Mycology: study of fungi; Parasitology: study of protozoa and parasitic worms; Immunology: study of immunity; Virology: study of viruses.

  • What is microbial genetics and molecular biology?

    Microbial genetics studies inheritance in microbes; molecular biology studies how genetic information is carried in DNA molecules.

  • What is genomics and recombinant DNA technology?

    Genomics studies an organism's genes; recombinant DNA technology combines DNA from different sources to produce proteins or hormones.

  • How do microbes benefit human welfare?

    Microbes recycle vital elements, treat sewage, clean pollutants, control insect pests, and produce useful products via biotechnology.

  • What are biofilms and their importance?

    Complex microbial communities attached to surfaces; can be beneficial (protect mucous membranes) or harmful (cause infections, resist antibiotics).

  • What factors contribute to emerging infectious diseases?

    Evolutionary changes like antibiotic resistance, modern transportation, and increased human exposure to infectious agents.