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BIO 175 Microbiology Midterm Study Guide: Chapters 1, 2, 3, 5, 7, 8, 9, 10, 14

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Introduction to Microbiology

Koch's Postulates

Members of the Bacterial World

Introduction to Bacteria

Introduction to Archaea

Introduction to Eukarya

Introduction to Staining

Simple Staining

Differential Staining

Gram Stain

Introduction to Microscopes

Light Microscopy: Bright-Field Microscopes

Light Microscopes that Increase Contrast

Light Microscopes that Detect Fluorescence

Electron Microscopes

Atoms- Smallest Unit of Matter

Isotopes

Introduction to Chemical Bonding

Covalent Bonds

Ionic Bonding

Hydrogen Bonding

Introduction to Water

Acids and Bases

pH Scale

Carbon

Functional Groups

Introduction to Biomolecules

Carbohydrates

Proteins

Nucleic Acids

Lipids

ATP

Enzymes

Introduction to Metabolism

Overview of Prokaryotic Cell Structure

Bacterial Cell Morphology & Arrangements

Introduction to Bacterial Cell Walls

Gram-Positive Cell Walls

Gram-Negative Cell Walls

Gram-Positive vs. Gram-Negative Cell Walls

The Glycocalyx: Capsules & Slime Layers

Introduction to Biofilms

Pili

Fimbriae & Hami

Introduction to Prokaryotic Flagella

Prokaryotic Flagellar Structure

Prokaryotic Flagellar Movement

Chemotaxis

Prokaryotic Ribosomes

Introduction to Bacterial Plasmids

Endospores

Sporulation

Germination

Binary Fission

Generation Times

Microbial Growth Curves in a Closed System

Temperature Requirements for Microbial Growth

Oxygen Requirements for Microbial Growth

pH Requirements for Microbial Growth

Nutritional Factors of Microbial Growth

Types of Solid Culture Media

Selective Media

Differential Media

Enrichment Media

Growing a Pure Culture

Plating Methods

Introduction to Cultivating Microbial Growth

Introduction to Controlling Microbial Growth

Physical Methods to Control Microbial Growth

Chemical Methods to Control Microbial Growth

Liquid Chemicals: Alcohols, Aldehydes, & Biguanides

Liquid Chemicals: Halogens

Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde

Introduction to Microbial Genetics

Introduction to Mutations

Methods of Inducing Mutations

Mutant Detection

The Ames Test

DNA Repair Mechanisms

Horizontal Gene Transfer

Bacterial Transformation

Transduction

Introduction to Conjugation

Conjugation: F Plasmids

Conjugation: Hfr & F' Cells

The Lac Operon

The Trp Operon

Review of the Lac Operon & Trp Operon

Central Dogma

Introduction to DNA Replication

DNA Polymerases

Leading & Lagging DNA Strands

Steps of DNA Replication

Introduction to Transcription

Steps of Transcription

Introduction to Translation

Steps of Translation

Prokaryotic Gene Expression

Introduction to Regulation of Gene Expression

Introduction to Viruses

Bacterial Pathogenesis

Introduction to Pathogenic Toxins

Exotoxins Cause Damage to the Host

Endotoxin Causes Damage to the Host

Exotoxins vs. Endotoxin Review

Introduction to Immunity

Introduction to Innate Immunity

Introduction to First-Line Defenses

Physical Barriers in First-Line Defenses: Skin

Physical Barriers in First-Line Defenses: Mucous Membrane

First-Line Defenses: Chemical Barriers

First-Line Defenses: Normal Microbiota

Introduction to Cells of the Immune System

Cells of the Immune System: Granulocytes

Cells of the Immune System: Agranulocytes

Introduction to Cell Communication

Cell Communication: Surface Receptors & Adhesion Molecules

Cell Communication: Cytokines

Pattern Recognition Receptors (PRRs)

Introduction to the Complement System

Activation Pathways of the Complement System

Effects of the Complement System

Phagoctytosis

Introduction to Inflammation

Steps of the Inflammatory Response

Fever

Interferon Response

Introduction to Adaptive Immunity

Antigens

Introduction to T Lymphocytes

Major Histocompatibility Complex Molecules

Activation of T Lymphocytes

Functions of T Lymphocytes

Review of Cytotoxic vs Helper T Cells

Introduction to B Lymphocytes

Antibodies

Classes of Antibodies

Outcomes of Antibody Binding to Antigen

T Dependent & T Independent Antigens

Clonal Selection

Antibody Class Switching

Affinity Maturation

Primary and Secondary Response of Adaptive Immunity

Immune Tolerance

Regulatory T Cells

Natural Killer Cells

Review of Adaptive Immunity

Introduction to Hypersensitivites

Type I Hypersensitivities

Type II Hypersensitivities

Type III Hypersensitivities

Type IV Hypersensitivities

Reviewing the 4 Types of Hypersensitivities

Autoimmune Diseases

Immunodeficiency Disorders

Types of Acquired Immunity

Introduction to Vaccines

Attenuated Vaccines

Inactivated Vaccines

Immunoassay: ELISA

Immunoassay: Western Blotting