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Comprehensive Study Guide: Recombinant DNA Technology, Microbial Control, Antimicrobial Drugs, Prokaryote Classification, Eukaryotes, Viruses, and Epidemiology

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Introduction to DNA-Based Technology

  • Map of DNA-Based Technology Lesson
    03:12
  • Introduction to DNA-Based Technology
    02:30

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

Gel Electrophoresis

  • Gel Electrophoresis
    07:58
  • Gel Electrophoresis Example 1
    02:34

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

CRISPR CAS

  • CRISPR CAS
    07:43

Southern Blotting

  • Southern Blotting
    03:31
  • Steps of Southern Blotting
    08:21

Introduction to Controlling Microbial Growth

  • Terminology of Microbial Growth Control
    07:36
  • Hierarchy of Microbial Growth Control Terminology
    03:09
  • Situations Warranting Different Levels of Microbial Growth Control
    05:58

Selecting a Method to Control Microbial Growth

  • Types of Microbes (Microbial Characteristics)
    07:24
  • Overall Risk of Infection
    04:52
  • Environmental Factors
    04:18

Physical Methods to Control Microbial Growth

  • Dry Heat
    02:58
  • Irradiation
    06:18
  • Filtration
    04:29

Chemical Methods to Control Microbial Growth

  • Types of Disinfectants
    05:21
  • Selecting the Right Chemical Agent
    04:52
  • Chemical Methods to Control Microbial Growth
    03:58

Chemicals Used to Control Microbial Growth

  • Chemicals Used to Control Microbial Growth
    02:29
  • Factors Affecting Chemical Agents
    03:17

Liquid Chemicals: Alcohols, Aldehydes, & Biguanides

  • Alcohols
    03:39
  • Biguanides
    02:41
  • Aldehydes
    03:59

Liquid Chemicals: Halogens

  • Iodine
    02:15
  • Chlorine
    03:16
  • Liquid Chemicals: Halogens
    00:56

Liquid Chemicals: Surface-Active Agents

  • Liquid Chemicals: Surface-Active Agents
    04:14
  • Soaps & Detergents
    06:20
  • Quats
    02:33

Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde

  • Ozone
    02:36
  • Formaldehyde Gas
    02:56
  • Chemical Gases: Ethylene Oxide
    03:22

Review of Chemicals Used to Control Microbial Growth

  • Review of Chemicals Used to Control Microbial Growth
    05:41

Introduction to Antimicrobial Drugs

  • Guided course
    History of Antimicrobial Drugs
    05:33

How Antimicrobial Drugs Work

  • Guided course
    Selective Toxicity
    05:27
  • Guided course
    How Antimicrobial Drugs Work Example 1
    03:28

Broad vs Narrow Spectrum Drugs

  • Guided course
    Broad vs Narrow Spectrum
    04:05

Superinfections

  • Guided course
    Superinfections Example 1
    01:59
  • Guided course
    Superinfections
    05:16

Drug Interactions: Synergism and Antagonism

  • Guided course
    Drug Synergism & Antagonism
    03:06
  • Guided course
    Drug Interactions: Synergism and Antagonism Example 1
    03:17

Therapeutic Window & Therapeutic Index

  • Guided course
    Therapeutic Window & Therapeutic Index
    04:08

Inhibitors of Cell Wall Synthesis: Beta-lactam & Penicillin

  • Guided course
    Inhibitors of Cell Wall Synthesis: Beta-lactam & Penicillin Example 3
    04:02
  • Guided course
    Other Beta-lactam Antibiotics
    04:51
  • Inhibitors of Cell Wall Synthesis: Beta-lactam & Penicillin Example 2
    02:21

Inhibitors of Protein Synthesis

  • Guided course
    Inhibitors of Protein Synthesis
    05:25
  • Guided course
    Inhibitors of Protein Synthesis Example 1
    03:04

Disruptors of Cell Membranes

  • Guided course
    Disruptors of Cell Membranes
    04:03

Inhibitors of Nucleic Acid Synthesis

  • Guided course
    Inhibitors of Nucleic Acid Synthesis Example 1
    02:21
  • Guided course
    Inhibitors of Nucleic Acid Synthesis
    05:01

Competitive Inhibitors of Metabolic Pathways

  • Guided course
    Competitive Inhibitors of Metabolic Pathways Example 1
    02:36
  • Guided course
    Sulfa Drugs & Folic Acid Synthesis
    05:22

Antifungal Drugs

  • Guided course
    Antifungal Drugs
    06:43
  • Guided course
    Antifungal Drugs Example 1
    02:06

Antiviral Drugs

  • Guided course
    Antiviral Drugs
    07:25

Tests to Guide Antimicrobial Use

  • Guided course
    Tests to Guide Antimicrobial Use Example 1
    04:17
  • Guided course
    Disk Diffusion Methods Including Kirby-Bauer Test
    06:04
  • Guided course
    Broth Dilution Test
    04:53

Antimicrobial Resistance

  • Guided course
    Types of Resistance Mechanisms
    05:04
  • Guided course
    Spread of Antibiotic Resistance
    04:45
  • Guided course
    Antibiotic Resistance
    03:45