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Comprehensive Study Guide: Recombinant DNA Technology, Microbial Control, Antimicrobial Drugs, Prokaryote Classification, Eukaryotes, Viruses, and Epidemiology

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Introduction to DNA-Based Technology

Introduction to DNA Cloning

Steps to DNA Cloning

Introduction to Polymerase Chain Reaction

The Steps of PCR

Gel Electrophoresis

Methods of Inducing Mutations

Introduction to DNA Repair

DNA Repair Mechanisms

Introduction to Microbial Genetics

Horizontal Gene Transfer

Bacterial Transformation

Transduction

Introduction to Conjugation

CRISPR CAS

Southern Blotting

Introduction to Controlling Microbial Growth

Selecting a Method to Control Microbial Growth

Physical Methods to Control Microbial Growth

Chemical Methods to Control Microbial Growth

Chemicals Used to Control Microbial Growth

Liquid Chemicals: Alcohols, Aldehydes, & Biguanides

Liquid Chemicals: Halogens

Liquid Chemicals: Surface-Active Agents

Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde

Review of Chemicals Used to Control Microbial Growth

Introduction to Antimicrobial Drugs

How Antimicrobial Drugs Work

Broad vs Narrow Spectrum Drugs

Superinfections

Drug Interactions: Synergism and Antagonism

Therapeutic Window & Therapeutic Index

Inhibitors of Cell Wall Synthesis: Beta-lactam & Penicillin

Inhibitors of Protein Synthesis

Disruptors of Cell Membranes

Inhibitors of Nucleic Acid Synthesis

Competitive Inhibitors of Metabolic Pathways

Antifungal Drugs

Antiviral Drugs

Tests to Guide Antimicrobial Use

Antimicrobial Resistance