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DNA Replication, Gene Expression, Mutations, and Molecular Genetics Techniques

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Discovering the Structure of DNA

  • Detailed DNA Structure
    06:24
  • Discovering the Structure of DNA
    04:41

Leading & Lagging DNA Strands

  • Leading & Lagging DNA Strands
    05:15

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

DNA Polymerases

  • DNA Polymerase Distinguishes Template from New Strand via Methylation
    03:47
  • DNA Polymerases Requirements
    05:03
  • DNA Polymerases
    04:01

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Eukaryotic RNA Processing and Splicing

  • Eukaryotic RNA Processing and Splicing
    02:32
  • 2) RNA Splicing Creates Mature mRNA
    08:44
  • 1) RNA Processing
    04:19

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • Elongation of Translation
    04:59
  • Termination of Translation
    02:16
  • Steps of Translation
    05:03

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Mutant Detection

  • Mutant Detection
    06:49
  • Replica Plating
    05:36

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

ATP

  • Energy Coupling
    04:57
  • ATP
    06:44
  • Phosphorylation
    03:00

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39